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CTLA4 antibody (Cytotoxic T-Lymphocyte-Associated Protein 4)

Details for Product anti-CTLA4 Antibody No. ABIN118890, Supplier: Login to see
Antigen
  • CTLA-4
  • CTLA4
  • CD152
  • Cd152
  • Ctla-4
  • Ly-56
  • CD
  • CELIAC3
  • GRD4
  • GSE
  • IDDM12
  • sCTLA4
Reactivity
Human
185
134
56
7
2
2
Host
Mouse
165
91
80
12
5
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CTLA4 antibody is un-conjugated
55
34
33
15
7
4
3
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
227
102
101
57
47
29
21
19
17
7
6
6
5
4
4
4
4
3
2
2
2
1
1
1
1
Supplier
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Immunogen Human CTLA-4/human IgG heavy chain fusion protein (CTLA-4/Ig)
Clone BNI3
Isotype IgG2a
Specificity Highly specific for CD152 (CTAL-4).
Alternative Name CD152 / CTLA4 (CTLA4 Antibody Abstract)
Background The cytolytic T-lymphocyte-associated antigen 4 (CTLA-4) is a member of the Ig superfamily (1) and its extracellular domain exhibits a single Ig V-like region. It was originally identified as a fourth cDNA product, during a search for genes that were thought to be specifically expressed in cytotoxic T lymphocytes (1). CTLA-4 has a molecular weight close to 45 kDa (2). It is mainly expressed as a monomer (4), but may also be expressed as a disulfide-linked homodimer (2,3). CTLA-4 belongs to the CD28 receptor family (4,5).Synonyms: CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4
Gene ID 1493
NCBI Accession NP_001032720
UniProt P16410
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways
Application Notes Research studies by Microscopy or Flow cytometry, after in vitro activation of T cells with selected stimuli (Concanavalin A, or solid-phase fixed-CD3 + CD28 Mabs stimulation). Research studies on CTLA-4 expression on B cells and on CTLA-4 expression by Immunohistochemistry only on frozen tissue sections. Fluorescence microscopy or Flow cytometry: 2 μg/5x10e5 cells/test. Cytospin or immunohistochemistry on frozen tissue sections: Working concentration is 5-50 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1 mL of distilled water.
Buffer PBS, pH 7.2, containing 1 mg/mL BSA as stabilizer without preservatives
Preservative Without preservative
Storage 4 °C
Storage Comment Store at 2 - 8 °C.
Background publications Castan, Tenner-Racz, Racz et al.: "Accumulation of CTLA-4 expressing T lymphocytes in the germinal centres of human lymphoid tissues." in: Immunology, Vol. 90, Issue 2, pp. 265-71, 1997 (PubMed).

Linsley, Golstein: "Lymphocyte activation: T-cell regulation by CTLA-4." in: Current biology : CB, Vol. 6, Issue 4, pp. 398-400, 1997 (PubMed).

Linsley, Bradshaw, Greene et al.: "Intracellular trafficking of CTLA-4 and focal localization towards sites of TCR engagement." in: Immunity, Vol. 4, Issue 6, pp. 535-43, 1996 (PubMed).

Morton, Fu, Stewart et al.: "Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 3, pp. 1047-54, 1996 (PubMed).

Lindsten, Lee, Harris et al.: "Characterization of CTLA-4 structure and expression on human T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3489-99, 1993 (PubMed).

Rudd, Janssen, Cai et al.: "Two-step TCR zeta/CD3-CD4 and CD28 signaling in T cells: SH2/SH3 domains, protein-tyrosine and lipid kinases." in: Immunology today, Vol. 15, Issue 5, pp. 225-34, 1994 (PubMed).

Gribben, Freeman, Boussiotis et al.: "CTLA4 mediates antigen-specific apoptosis of human T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 3, pp. 811-5, 1995 (PubMed).

Kuiper, Brouwer, Linsley et al.: "Activated T cells can induce high levels of CTLA-4 expression on B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 4, pp. 1776-83, 1995 (PubMed).

Linsley, Nadler, Bajorath et al.: "Binding stoichiometry of the cytotoxic T lymphocyte-associated molecule-4 (CTLA-4). A disulfide-linked homodimer binds two CD86 molecules." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15417-24, 1995 (PubMed).

June, Bluestone, Nadler et al.: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994 (PubMed).

Waterhouse, Penninger, Timms et al.: "Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4." in: Science (New York, N.Y.), Vol. 270, Issue 5238, pp. 985-8, 1995 (PubMed).

Brunet, Denizot, Luciani et al.: "A new member of the immunoglobulin superfamily--CTLA-4." in: Nature, Vol. 328, Issue 6127, pp. 267-70, 1987 (PubMed).

Linsley, Brady, Urnes et al.: "CTLA-4 is a second receptor for the B cell activation antigen B7." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 561-9, 1991 (PubMed).

Harper, Balzano, Rouvier et al.: "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 3, pp. 1037-44, 1991 (PubMed).

Linsley, Greene, Tan et al.: "Coexpression and functional cooperation of CTLA-4 and CD28 on activated T lymphocytes." in: The Journal of experimental medicine, Vol. 176, Issue 6, pp. 1595-604, 1993 (PubMed).

Freeman, Lombard, Gimmi et al.: "CTLA-4 and CD28 mRNA are coexpressed in most T cells after activation. Expression of CTLA-4 and CD28 mRNA does not correlate with the pattern of lymphokine production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 12, pp. 3795-801, 1993 (PubMed).