Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody

Details for Product No. ABIN118890
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Antigen
Synonyms CTLA-4, CTLA4, CD152, Cd152, Ctla-4, Ly-56, CD, CELIAC3, GRD4, GSE, IDDM12, sCTLA4
Reactivity
Human
(128), (105), (74), (17), (17), (2)
Host
Mouse
(100), (79), (58), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(27), (26), (20), (7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Functional Studies (Func), Flow Cytometry (FACS)
(150), (70), (64), (34), (20), (20), (18), (15), (13), (11), (10), (2), (2), (1), (1)
Pubmed 16 references available
Quantity 0.2 mg
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Request Want additional data for this product?

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Catalog No. ABIN118890
643.50 $
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Immunogen Human CTLA-4/human IgG heavy chain fusion protein (CTLA-4/Ig)
Clone BNI3
Isotype IgG2a
Specificity Highly specific for CD152 (CTAL-4).
Alternative Name CD152 / CTLA4
Background The cytolytic T-lymphocyte-associated antigen 4 (CTLA-4) is a member of the Ig superfamily (1) and its extracellular domain exhibits a single Ig V-like region. It was originally identified as a fourth cDNA product, during a search for genes that were thought to be specifically expressed in cytotoxic T lymphocytes (1). CTLA-4 has a molecular weight close to 45kDa (2). It is mainly expressed as a monomer (4), but may also be expressed as a disulfide-linked homodimer (2,3). CTLA-4 belongs to the CD28 receptor family (4,5).
Alternate names: CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4
Gene ID 1493
NCBI Accession NP_001032720
UniProt P16410
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Research studies by Microscopy or Flow cytometry, after in vitro activation of T cells with selected stimuli (Concanavalin A, or solid-phase fixed-CD3 + CD28 Mabs stimulation). Research studies on CTLA-4 expression on B cells and on CTLA-4 expression by Immunohistochemistry only on frozen tissue sections. Fluorescence microscopy or Flow cytometry: 2 ug/5x10e5 cells/test. Cytospin or immunohistochemistry on frozen tissue sections: Working concentration is 5-50 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1 mL of distilled water.
Buffer PBS, pH 7.2, containing 1 mg/ml BSA as stabilizer without preservatives
Preservative Without preservative
Storage 4 °C
Storage Comment Store at 2 - 8 °C.
Expiry Date 12 months
Background publications Freeman, Lombard, Gimmi et al.: "CTLA-4 and CD28 mRNA are coexpressed in most T cells after activation. Expression of CTLA-4 and CD28 mRNA does not correlate with the pattern of lymphokine production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 12, pp. 3795-801, 1993 (PubMed).

Linsley, Greene, Tan et al.: "Coexpression and functional cooperation of CTLA-4 and CD28 on activated T lymphocytes." in: The Journal of experimental medicine, Vol. 176, Issue 6, pp. 1595-604, 1993 (PubMed).

Harper, Balzano, Rouvier et al.: "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 3, pp. 1037-44, 1991 (PubMed).

Linsley, Brady, Urnes et al.: "CTLA-4 is a second receptor for the B cell activation antigen B7." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 561-9, 1991 (PubMed).

Brunet, Denizot, Luciani et al.: "A new member of the immunoglobulin superfamily--CTLA-4." in: Nature, Vol. 328, Issue 6127, pp. 267-70, 1987 (PubMed).

Waterhouse, Penninger, Timms et al.: "Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4." in: Science (New York, N.Y.), Vol. 270, Issue 5238, pp. 985-8, 1995 (PubMed).

June, Bluestone, Nadler et al.: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994 (PubMed).

Linsley, Nadler, Bajorath et al.: "Binding stoichiometry of the cytotoxic T lymphocyte-associated molecule-4 (CTLA-4). A disulfide-linked homodimer binds two CD86 molecules." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15417-24, 1995 (PubMed).

Kuiper, Brouwer, Linsley et al.: "Activated T cells can induce high levels of CTLA-4 expression on B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 4, pp. 1776-83, 1995 (PubMed).

Gribben, Freeman, Boussiotis et al.: "CTLA4 mediates antigen-specific apoptosis of human T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 3, pp. 811-5, 1995 (PubMed).

Rudd, Janssen, Cai et al.: "Two-step TCR zeta/CD3-CD4 and CD28 signaling in T cells: SH2/SH3 domains, protein-tyrosine and lipid kinases." in: Immunology today, Vol. 15, Issue 5, pp. 225-34, 1994 (PubMed).

Lindsten, Lee, Harris et al.: "Characterization of CTLA-4 structure and expression on human T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3489-99, 1993 (PubMed).

Morton, Fu, Stewart et al.: "Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 3, pp. 1047-54, 1996 (PubMed).

Linsley, Bradshaw, Greene et al.: "Intracellular trafficking of CTLA-4 and focal localization towards sites of TCR engagement." in: Immunity, Vol. 4, Issue 6, pp. 535-43, 1996 (PubMed).

Linsley, Golstein: "Lymphocyte activation: T-cell regulation by CTLA-4." in: Current biology : CB, Vol. 6, Issue 4, pp. 398-400, 1997 (PubMed).

Castan, Tenner-Racz, Racz et al.: "Accumulation of CTLA-4 expressing T lymphocytes in the germinal centres of human lymphoid tissues." in: Immunology, Vol. 90, Issue 2, pp. 265-71, 1997 (PubMed).

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