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Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody

Antigen

Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4)
Synonyms CD, GSE, ICOS, CD152, CTLA-4, IDDM12, CELIAC3, Cd152, Ly-56, Ctla-4, sCTLA4, CTLA4
Clonality Monoclonal (BNI3)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Functional Studies (Func), Flow Cytometry (FACS)
10 references available
Catalog no. ABIN118890
Quantity 0.2 mg  (n. a.)  (Variants)
Price 616.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name CD152 / CTLA4
UniProt P16410
Immunogen Human CTLA-4/human IgG heavy chain fusion protein (CTLA-4/Ig)
Cross-Reactivity Human
Isotype IgG2a
Clone BNI3
Description The cytolytic T-lymphocyte-associated antigen 4 (CTLA-4) is a member of the Ig superfamily (1) and its extracellular domain exhibits a single Ig V-like region. It was originally identified as a fourth cDNA product, during a search for genes that were thought to be specifically expressed in cytotoxic T lymphocytes (1). CTLA-4 has a molecular weight close to 45kDa (2). It is mainly expressed as a monomer (4), but may also be expressed as a disulfide-linked homodimer (2,3). CTLA-4 belongs to the CD28 receptor family (4,5). Alternate Names: CTLA4, CTLA-4, Cytotoxic T-lymphocyte-associated antigen 4, Cytotoxic T-lymphocyte protein 4.

Application Details
Publications Alternatives Variants back to top

Application Notes Research studies by Microscopy or Flow cytometry, after in vitro activation of T cells with selected stimuli (Concanavalin A, or solid-phase fixed-CD3 + CD28 Mabs stimulation). Research studies on CTLA-4 expression on B cells and on CTLA-4 expression by Immunohistochemistry only on frozen tissue sections. Fluorescence microscopy or Flow cytometry: 2 ug/5x10e5 cells/test. Cytospin or immunohistochemistry on frozen tissue sections: Working concentration is 5-50 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration n. a.
Buffer PBS, pH 7.2, containing 1 mg/ml BSA as stabilizer without preservatives.
Preservative Azide Free
Storage Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: one year from despatch.Reconstitution: Restore with 1 ml of distilled water..
Research Area CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Linsley, Greene, Tan et al.: "Coexpression and functional cooperation of CTLA-4 and CD28 on activated T lymphocytes." in: The Journal of experimental medicine, Vol. 176, Issue 6, pp. 1595-604, 1993 (PubMed).

Harper, Balzano, Rouvier et al.: "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 3, pp. 1037-44, 1991 (PubMed).

Linsley, Brady, Urnes et al.: "CTLA-4 is a second receptor for the B cell activation antigen B7." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 561-9, 1991 (PubMed).

Brunet, Denizot, Luciani et al.: "A new member of the immunoglobulin superfamily--CTLA-4." in: Nature, Vol. 328, Issue 6127, pp. 267-70, 1987 (PubMed).

June, Bluestone, Nadler et al.: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994 (PubMed).

Linsley, Nadler, Bajorath et al.: "Binding stoichiometry of the cytotoxic T lymphocyte-associated molecule-4 (CTLA-4). A disulfide-linked homodimer binds two CD86 molecules." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15417-24, 1995 (PubMed).

Rudd, Janssen, Cai et al.: "Two-step TCR zeta/CD3-CD4 and CD28 signaling in T cells: SH2/SH3 domains, protein-tyrosine and lipid kinases." in: Immunology today, Vol. 15, Issue 5, pp. 225-34, 1994 (PubMed).

Lindsten, Lee, Harris et al.: "Characterization of CTLA-4 structure and expression on human T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3489-99, 1993 (PubMed).

Morton, Fu, Stewart et al.: "Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 3, pp. 1047-54, 1996 (PubMed).

Castan, Tenner-Racz, Racz et al.: "Accumulation of CTLA-4 expressing T lymphocytes in the germinal centres of human lymphoid tissues." in: Immunology, Vol. 90, Issue 2, pp. 265-71, 1997 (PubMed).