CTLA4 antibody

Catalog No. ABIN2749015

Cited in 4+ publications. The Mouse Monoclonal anti-CTLA4 antibody (Clone BNI3) (ABIN2749015) specifically detects CTLA4 in FACS, IP, ICC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for CTLA4 antibody (ABIN2749015)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BNI3

Product Details anti-CTLA4 Antibody

Purpose: Anti-Hu CD152 Purified Low Endotoxin

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody BNI3 recognizes an extracellular domain of human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human CD152-IgG heavy chain fusion protein

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL, Intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CTLA4 antibody (ABIN2749015)

Kraszula, Eusebio, Kupczyk, Kuna, Pietruczuk: "[The use of multi-color flow cytometry for identification of functional markers of nTregs in patients with severe asthma]." in: Pneumonologia i alergologia polska, Vol. 80, Issue 5, pp. 389-401, (2012) (PubMed).

Chin, Chu, Chen, Hsu, Weng, Chu: "Site-directed in vitro immunization leads to a complete human monoclonal IgG4 lambda that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands." in: BMC biotechnology, Vol. 7, pp. 51, (2007) (PubMed).

Steiner, Moosig, Csernok, Selleng, Gross, Fleischer, Bröker: "Increased expression of CTLA-4 (CD152) by T and B lymphocytes in Wegener's granulomatosis." in: Clinical and experimental immunology, Vol. 126, Issue 1, pp. 143-50, (2001) (PubMed).

Steiner, Waase, Rau, Dietrich, Fleischer, Bröker: "Enhanced expression of CTLA-4 (CD152) on CD4+ T cells in HIV infection." in: Clinical and experimental immunology, Vol. 115, Issue 3, pp. 451-7, (1999) (PubMed).

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152

Background: Cytotoxic T-lymphocyte associated protein 4,CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.,CTLA4, GSE, GRD4

Gene ID: 1493

UniProt: P16410

Pathways: Cancer Immune Checkpoints