HLA Class I ABC antibody

Details for Product No. ABIN118899, Supplier: Log in to see
Baboon, Cow (Bovine), Chimpanzee, Cat (Feline), Human, Macaque, Monkey, Primate
Clonality (Clone)
Monoclonal ()
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)
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Immunogen Purified Human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line
Clone W6-32
Isotype IgG2a
Purification Affinity Chromatography on Protein G
Endotoxin Level Less than 0.01 EU/μg
Background Synonyms: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I
Research Area Major Histocompatibility Complex (MHC)
Application Notes This product is routinely tested in flow cytometry on human peripheral blood lymphocytes: Use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections (see Ref 6, Tonsil is useful as positive control). Not suitable for Immunohistochemistry on paraffin sections. Immunoprecipitation (Ref 1). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS without preservatives or stabilizers.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
 image for anti-HLA Class I ABC antibody (ABIN118899) Staining of peripheral blood lymphocytes with anti-HLA-ABC: Endotoxin low (ABIN118899).
Product cited in: Stern, Goldman, Hatasaka, Mackenzie, Surrey, Racowsky,: "Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology database study." in: Fertility and sterility, 2008 (PubMed).

Mayr, Bund, Schlee, Moosmann, Kofler, Hallek, Wendtner: "Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes." in: Blood, Vol. 105, Issue 4, pp. 1566-73, 2005 (PubMed).

Mayr, Kofler, Büning, Bund, Hallek, Wendtner: "Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells." in: Blood, Vol. 106, Issue 9, pp. 3223-6, 2005 (PubMed).

Background publications Ishitani, Sageshima, Lee, Dorofeeva, Hatake, Marquardt, Geraghty: "Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1376-84, 2003 (PubMed).

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000 (PubMed).

Verbeek, Westphal, Ruiter, de Waal: "T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5876-84, 1995 (PubMed).

Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, 1994 (PubMed).

Tanabe, Sekimata, Ferrone, Takiguchi: "Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 10, pp. 3202-9, 1992 (PubMed).

Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, 1987 (PubMed).

Barnstable, Bodmer, Brown, Galfre, Milstein, Williams, Ziegler: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." in: Cell, Vol. 14, Issue 1, pp. 9-20, 1978 (PubMed).