HLA-ABC antibody

Catalog No. ABIN118899

Product Details anti-HLA-ABC Antibody

Target: HLA-ABC

Reactivity: Human, Cow, Baboon, Cat, Monkey, Macaque, Primate, Chimpanzee

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-ABC antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Functional Studies (Func), Enzyme Immunoassay (EIA), DNA Microscopy (DNA Mic)

Purification: Affinity Chromatography on Protein G

Endotoxin Level: Less than 0.01 EU/μg

Immunogen: Purified Human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line

Clone: W6-32

Isotype: IgG2a

Application Details

Application Notes: This product is routinely tested in flow cytometry on human peripheral blood lymphocytes: Use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections (see Ref 6, Tonsil is useful as positive control). Not suitable for Immunohistochemistry on paraffin sections. Immunoprecipitation (Ref 1). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives or stabilizers.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-HLA-ABC antibody (ABIN118899)

Giersch, Hermanussen, Volz, Volmari, Allweiss, Sureau, Casey, Huang, Fischer, Lütgehetmann, Dandri: "Strong Replication Interference Between Hepatitis Delta Viruses in Human Liver Chimeric Mice." in: Frontiers in microbiology, Vol. 12, pp. 671466, (2021) (PubMed).

Stern, Goldman, Hatasaka, Mackenzie, Surrey, Racowsky,: "Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology database study." in: Fertility and sterility, (2008) (PubMed).

Mayr, Bund, Schlee, Moosmann, Kofler, Hallek, Wendtner: "Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes." in: Blood, Vol. 105, Issue 4, pp. 1566-73, (2005) (PubMed).

Mayr, Kofler, Büning, Bund, Hallek, Wendtner: "Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells." in: Blood, Vol. 106, Issue 9, pp. 3223-6, (2005) (PubMed).

Target Details for HLA-ABC

Target: HLA-ABC

Alternative Name: HLA Class I ABC (HLA-ABC Products)

Synonyms: HLAA antibody, major histocompatibility complex, class I, A antibody, HLA-A antibody

Background: Synonyms: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints