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Details for Product No. ABIN119332

Toll-Like Receptor 4 (TLR4) antibody

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Antigen
Synonyms ARMD10, CD284, TLR-4, TOLL, Lps, Ly87, Ran/M1, Rasl2-8
Reactivity
»Alternatives Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
Pubmed 9 references available
Catalog no. ABIN119332
Quantity 0.1 mg
Price
242.00 $   Plus shipping costs $45.00
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Immunogen Ba/F3 cell line expressing TLR4. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone HTA125
Isotype IgG2a
Specificity This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
Purification Affinity Chromatography on Protein G
Alternative Name CD284 / TLR4
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Alternate names: Toll-like receptor 4
NCBI Accession NM_138554.3
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Application Notes Flow cytometry: 1/10-1/25, use 10 ul of the suggested working solution to label 10e6 cells or 100ul whole blood. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Yang, Tamai, Akashi et al.: "Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture." in: Infection and immunity, Vol. 69, Issue 4, pp. 2045-53, 2001 (PubMed).

Kawahara, Teshima, Oka et al.: "Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells." in: Infection and immunity, Vol. 69, Issue 7, pp. 4382-9, 2001 (PubMed).

Triantafilou, Miyake, Golenbock et al.: "Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation." in: Journal of cell science, Vol. 115, Issue Pt 12, pp. 2603-11, 2002 (PubMed).

Bieback, Lien, Klagge et al.: "Hemagglutinin protein of wild-type measles virus activates toll-like receptor 2 signaling." in: Journal of virology, Vol. 76, Issue 17, pp. 8729-36, 2002 (PubMed).

Devaney, Greene, Taggart et al.: "Neutrophil elastase up-regulates interleukin-8 via toll-like receptor 4." in: FEBS letters, Vol. 544, Issue 1-3, pp. 129-32, 2003 (PubMed).

Brännström, Sellin, Holmfeldt et al.: "The Schistosoma mansoni protein Sm16/SmSLP/SmSPO-1 assembles into a nine-subunit oligomer with potential To inhibit Toll-like receptor signaling." in: Infection and immunity, Vol. 77, Issue 3, pp. 1144-54, 2009 (PubMed).

de Kleer, Vercoulen, Klein et al.: "CD30 discriminates heat shock protein 60-induced FOXP3+ CD4+ T cells with a regulatory phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 4, pp. 2071-9, 2010 (PubMed).

Alternatives for antigen "Toll-Like Receptor 4 (TLR4)", type "Antibodies"
Hosts (140), (127), (20), (5)
Reactivities (222), (89), (35), (24), (23), (21), (18), (11), (4), (3), (2), (2), (1), (1), (1)
Applications (149), (141), (64), (61), (43), (32), (29), (18), (17), (10), (9), (7), (6), (3), (2), (1), (1), (1), (1)
Conjugates (30), (28), (18), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (23), (13), (9), (9), (7), (7), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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