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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Toll-Like Receptor 4 (TLR4) antibody
|Synonyms||TOLL, CD284, hToll, ARMD10, Lps, Ly87, Ran/M1, Rasl2-8|
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
|Quantity||0.1 mg (1.0 mg/ml) (Variants)|
|Price||236.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||CD284 / TLR4|
|Immunogen||Ba/F3 cell line expressing TLR4. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line|
|Cross-Reactivity||Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)|
|Description||TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Alternate Names: TLR-4, hToll, Toll-like Receptor 4.|
|Specificity||This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of ABIN119330 for this purpose.|
|Application Notes||Flow cytometry: 1/10-1/25, use 10 ul of the suggested working solution to label 10e6 cells or 100ul whole blood. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.09% Sodium Azide|
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Research Area||Signaling, Immunology, Inflammation|
|Restrictions||For Research Use only|
Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).
Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).
Yang, Tamai, Akashi et al.: "Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture." in: Infection and immunity, Vol. 69, Issue 4, pp. 2045-53, 2001 (PubMed).