beta-2 Microglobulin antibody
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- Target See all beta-2 Microglobulin (B2M) Antibodies
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This beta-2 Microglobulin antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognises beta-2 microglobulin, expressed at the surface of most cells in association with MHC Class I molecules.
- Purification
- Affinity chromatography on Protein G
- Immunogen
- Rat microglial cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse MOM myeloma cell line.
- Clone
- TLD-3H12B
- Isotype
- IgG1
- Top Product
- Discover our top product B2M Primary Antibody
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anti-beta-2-Microglobulin (B2M) (AA 21-100) antibody
B2M Reactivity: Human WB, FACS, ELISA, IHC Host: Mouse Monoclonal 3G5H8 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 1-119) antibodyB2M Reactivity: Human WB, ELISA, IF, IHC (p), RNAi, PLA Host: Mouse Monoclonal 3F9-2C2 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human WB, FACS, ELISA, IP, IHC (p), ICC, RIA Host: Mouse Monoclonal B2M-01 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M Reactivity: Mouse WB, IHC, IP, ICC Host: Mouse Monoclonal A2 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human, Non-Human Primate FACS, IHC, IF, StM Host: Mouse Monoclonal rB2M-961 unconjugated Recombinant Antibody
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human, Non-Human Primate IHC, StM Host: Mouse Monoclonal B2M-1118 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human, Non-Human Primate WB, FACS, IHC, IF, StM Host: Mouse Monoclonal B2M-961 unconjugated
anti-beta-2-Microglobulin (B2M) antibody (Biotin)B2M Reactivity: Human WB, FACS, ELISA, IP, IHC (p), ICC Host: Mouse Monoclonal B2M-01 Biotin
anti-beta-2-Microglobulin (B2M) antibodyB2M Reactivity: Human, Non-Human Primate FACS, IF, IHC (p) Host: Mouse Monoclonal B2M-961 unconjugated
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- Application Notes
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Flow cytometry: 1/10 - 1/20, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
- Alternative Name
- beta-2-Microglobulin (B2M Products)
- Background
- Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin
- Gene ID
- 24223
- NCBI Accession
- NP_036644
- UniProt
- P07151
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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