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beta-2 Microglobulin antibody (Biotin)

B2M Reactivity: Human WB, FACS, ELISA, IP, IHC (p), ICC Host: Mouse Monoclonal B2M-01 Biotin
Catalog No. ABIN93904
  • Target See all beta-2 Microglobulin (B2M) Antibodies
    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
    Reactivity
    • 204
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    • 27
    • 9
    • 4
    • 4
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    Human
    Host
    • 118
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    Mouse
    Clonality
    • 133
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    Monoclonal
    Conjugate
    • 112
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    This beta-2 Microglobulin antibody is conjugated to Biotin
    Application
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    Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Specificity
    The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M, extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
    No Cross-Reactivity
    Chicken, Cow, Dog, Mouse, Rabbit
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
    Immunogen
    Purified human beta2-microglobulin
    Clone
    B2M-01
    Isotype
    IgG2a
    Top Product
    Discover our top product B2M Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 1-2 μg/mL, positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.
    Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions.
    Comment

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).

    Khurana, Traum, Aivado, Wells, Guerrero, Grall, Libermann, Schachter: "Urine proteomic profiling of pediatric nephrotic syndrome." in: Pediatric nephrology (Berlin, Germany), Vol. 21, Issue 9, pp. 1257-65, (2006) (PubMed).

    Lafon, Prehaud, Megret, Lafage, Mouillot, Roa, Moreau, Rouas-Freiss, Carosella: "Modulation of HLA-G expression in human neural cells after neurotropic viral infections." in: Journal of virology, Vol. 79, Issue 24, pp. 15226-37, (2005) (PubMed).

    Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, (1985) (PubMed).

  • Target
    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
    Alternative Name
    beta2-Microglobulin (B2M Products)
    Synonyms
    Ly-m11 antibody, beta2-m antibody, beta2m antibody, bwm antibody, wu:fa94c05 antibody, wu:fb10a09 antibody, b2m-W01 antibody, b2m-W03 antibody, b2m-Z01 antibody, b2m-Z02 antibody, b2m-Z03 antibody, B2M antibody, b2m antibody, B2MG antibody, wu:fb66f11 antibody, wu:fj01f08 antibody, zgc:64161 antibody, b-2-m antibody, beta-2-microglobulin antibody, beta-2 microglobulin antibody, beta-2-microglobulin S homeolog antibody, beta-2-microglobulin, like antibody, B2M antibody, B2m antibody, b2m antibody, b2m.S antibody, b2ml antibody
    Background
    Beta-2-microglobulin,Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.,B2M
    Gene ID
    567
    UniProt
    P61769
    Pathways
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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