LRP1 antibody

Catalog No. ABIN119413

Product Details anti-LRP1 Antibody

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LRP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognises CD91, a type I membrane protein which is also known as the alpha 2 macroglobulin receptor (alpha 2MR). This antibody recognises an epitope in the extracellular region of the 500 kDa alpha chain.

Purification: Affinity chromatography on Protein G

Immunogen: Purified alpha2 macroglobulin receptor. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.

Clone: Alpha2MRalpha2

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Western Blot. ELISA. Immunohistochemistry on frozen sections. Recommended positive control tissue: Liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LRP1

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Alternative Name: CD91 / LRP1 (LRP1 Products)

Background: Low density lipoprotein receptor-related protein (also known as low density LRP, LRP1, alpha-2-macroglobulin receptor or Apolipoprotein E receptor) is an endocytotic receptor that is involved both in endocytosis and in phagocytosis of apoptotic cells. It is required for early embryonic development, is involved in cellular lipid homeostasis, and may play a role in APP metabolism, kinase-dependent intracellular signalling, neuronal calcium signalling and neurotransmission. Low density LRP also plays a role in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha-2-macroglobulin), and is involved in the local metabolism of complexes of plasminogen activators and their endogenous ligands. Low density LPR is postulated to be one of the major players in host resistance to HIV. The precursor low density LRP molecule is cleaved post-translationally to form a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515), which remains non-covalently associated with LRP-85. Following cleavage, the intracellular domain (LPRICD) is present in both the cytoplasm and the nucleus.Synonyms: A2MR, APOER, APR, Alpha-2-macroglobulin receptor, Apolipoprotein E receptor, Prolow-density lipoprotein receptor-related protein 1

Gene ID: 4035

NCBI Accession: NP_002323

UniProt: Q07954

Pathways: Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling