MCAM antibody (N-Term)
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- Target See all MCAM Antibodies
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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Binding Specificity
- AA 1-5, N-Term
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MCAM antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody recognizes the 118 kDa cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumor progression.
- Purification
- Affinity chromatography on Protein G
- Immunogen
- Recombinant human MUC18 (D1-D5) Fc protein. Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.
- Clone
- OJ79c
- Isotype
- IgG1
- Top Product
- Discover our top product MCAM Primary Antibody
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anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 84-189) antibody
MCAM Reactivity: Human FACS, IHC, WB, ELISA Host: Mouse Monoclonal 6C3E6 unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 50-646) antibodyMCAM Reactivity: Human FACS, WB, IF, ELISA Host: Mouse Monoclonal 4D10C7 unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 447-646) antibodyMCAM Reactivity: Human IHC, WB, IF Host: Rabbit Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM Reactivity: Human FACS, IHC, WB, IF, ELISA Host: Rabbit Monoclonal 8A10 unconjugated Recombinant Antibody
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 226-374) antibodyMCAM Reactivity: Human IHC, StM Host: Mouse Monoclonal MCAM-3046 unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 1-646) antibodyMCAM Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM Reactivity: Human, Mouse, Rat IHC, WB Host: Rabbit Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM Reactivity: Human, Mouse, Dog, Rabbit FACS, WB, IHC (p), IP, IHC (fro), ICC Host: Mouse Monoclonal P1H12 unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 201-300) antibodyMCAM Reactivity: Human, Rat FACS, WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Flow cytometry: 1/10 - 1/50, use 10 μL of the suggested working dilution to label 106 cells. Immunohistochemistry on frozen sections. Recommended positive control tissue: Melanoma. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, None, None
- Preservative
- Azide free
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody at -20 °C only.
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- Target
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- Alternative Name
- CD146 (MCAM Products)
- Background
- CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumour progression.Synonyms: A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen
- Gene ID
- 4162
- NCBI Accession
- NP_006491
- UniProt
- P43121
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