MCAM antibody

Catalog No. ABIN2749195

Cited in 3+ publications. The Mouse Monoclonal anti-MCAM antibody (Clone P1H12) (ABIN2749195) specifically detects MCAM in FACS, WB, IP, IHC (p), ICC and IHC (fro). The antibody is reactive with Human, Mouse, Rabbit and Dog samples.

Quick Overview for MCAM antibody (ABIN2749195)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human, Mouse, Rabbit, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: P1H12

Product Details anti-MCAM Antibody

Purpose: Anti-Hu CD146 Purified

No Cross-Reactivity: Rat

Cross-Reactivity (Details): Human, Mouse, Canine (Dog), Rabbit

Characteristics: The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: cultured human umbilical cells

Isotype: IgG1

Application Details

Application Notes: Western blotting: Nonreducing conditions.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-MCAM antibody (ABIN2749195)

Kamstock, Guth, Elmslie, Kurzman, Liggitt, Coro, Fairman, Dow: "Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma." in: Cancer gene therapy, Vol. 13, Issue 3, pp. 306-17, (2006) (PubMed).

Solovey, Gui, Chang, Enenstein, Browne, Hebbel: "Identification and functional assessment of endothelial P1H12." in: The Journal of laboratory and clinical medicine, Vol. 138, Issue 5, pp. 322-31, (2001) (PubMed).

Solovey, Lin, Browne, Choong, Wayner, Hebbel: "Circulating activated endothelial cells in sickle cell anemia." in: The New England journal of medicine, Vol. 337, Issue 22, pp. 1584-90, (1997) (PubMed).

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: Melanoma cell adhesion molecule,CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50 % of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.,MelCAM, MCAM, MUC18

Gene ID: 4162

UniProt: P43121