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MCAM antibody (Melanoma Cell Adhesion Molecule) Primary Antibody

MCAM Reactivity: Dog, Human, Mouse, Rabbit IHC (f), ICC, FACS, IP, IHC (p), WB Host: Mouse Monoclonal P1H12
Pubmed (3)
Catalog No. ABIN2749195
$364.32
Plus shipping costs $45.00
0.1 mg
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  • Target
    Reactivity
    Dog, Human, Mouse, Rabbit
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This MCAM antibody is un-conjugated
    Application
    Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    The mouse monoclonal antibody P1H12 recognizes CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.
    Workshop: HLDA 8
    No Cross-Reactivity
    Rat (Rattus)
    Cross-Reactivity (Details)
    Human, Mouse, Canine (Dog), Rabbit
    Purification
    Purified by protein-A affinity chromatography
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    cultured human umbilical cells
    Clone
    P1H12
    Isotype
    IgG1
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Kamstock, Guth, Elmslie, Kurzman, Liggitt, Coro, Fairman, Dow: "Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma." in: Cancer gene therapy, Vol. 13, Issue 3, pp. 306-17, 2006 (PubMed).

    Solovey, Gui, Chang, Enenstein, Browne, Hebbel: "Identification and functional assessment of endothelial P1H12." in: The Journal of laboratory and clinical medicine, Vol. 138, Issue 5, pp. 322-31, 2001 (PubMed).

    Solovey, Lin, Browne, Choong, Wayner, Hebbel: "Circulating activated endothelial cells in sickle cell anemia." in: The New England journal of medicine, Vol. 337, Issue 22, pp. 1584-90, 1997 (PubMed).

  • Target
    Alternative Name
    CD146 (MCAM Antibody Abstract)
    Synonyms
    CD146, MUC18, Muc18, 1-gicerin, AV025631, CD149, s-endo, s-gicerin, cd146, gicerin, id:ibd3512, mcam, MCAM, melanoma cell adhesion molecule, melanoma cell adhesion molecule b, MCAM, Mcam, mcamb
    Background
    CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50 % of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.
    Gene ID
    4162
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