MCAM antibody (Melanoma Cell Adhesion Molecule)

Catalog No. ABIN2749195

Product Details anti-MCAM Antibody

Target:

MCAM

Reactivity: Dog, Human, Mouse, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Specificity: The mouse monoclonal antibody P1H12 recognizes CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.
Workshop: HLDA 8

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Human, Mouse, Canine (Dog), Rabbit

Purification: Purified by protein-A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: cultured human umbilical cells

Clone: P1H12

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-MCAM antibody (ABIN2749195)

Kamstock, Guth, Elmslie, Kurzman, Liggitt, Coro, Fairman, Dow: "Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma." in: Cancer gene therapy, Vol. 13, Issue 3, pp. 306-17, 2006 (PubMed).

Solovey, Gui, Chang, Enenstein, Browne, Hebbel: "Identification and functional assessment of endothelial P1H12." in: The Journal of laboratory and clinical medicine, Vol. 138, Issue 5, pp. 322-31, 2001 (PubMed).

Solovey, Lin, Browne, Choong, Wayner, Hebbel: "Circulating activated endothelial cells in sickle cell anemia." in: The New England journal of medicine, Vol. 337, Issue 22, pp. 1584-90, 1997 (PubMed).

Target Details for MCAM

Target:

MCAM

Alternative Name: CD146 (MCAM Antibody Abstract)

Synonyms: CD146, MUC18, Muc18, 1-gicerin, AV025631, CD149, s-endo, s-gicerin, cd146, gicerin, id:ibd3512, mcam, MCAM, melanoma cell adhesion molecule, melanoma cell adhesion molecule b, MCAM, Mcam, mcamb

Background: CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50 % of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.

Gene ID: 4162