CD34 antibody (FITC)

Catalog No. ABIN120286

Product Details anti-CD34 Antibody (FITC)

Target: CD34

Reactivity: Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: SM2257F is specific for Canine CD34, a cell surface antigen of approximately 110 kDa expressed by endothelial cells and haematopoietic stem cells. Clone 1H6 is reported for use in CD34+ enrichment studies (4,5).

Purification: Affinity Chromatography on Protein G.

Immunogen: Canine CD34 fusion protein. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1/FOX-NY myeloma cell line.

Clone: 1H6

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (neat - 1/10): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 415130

NCBI Accession: NP_001003341

UniProt: Q28270