CD200 antibody

Catalog No. ABIN120528

Product Details anti-CD200 Antibody

Target: CD200

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD200 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises the rat CD200 cell surface antigen, also known as OX-2. This product is routinely tested by flow cytometry on rat thymocytes.

Purification: Affinity chromatography on Protein G

Immunogen: Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.

Clone: OX-2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, we recommend the use of SM294Afor use in functional studies. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD200

Target: CD200

Alternative Name: CD200 (CD200 Products)

Background: CD200 is a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells. Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation.Synonyms: MOX1, MOX2, My033, OX-2 membrane glycoprotein

Gene ID: 24560

NCBI Accession: NP_113706

UniProt: P04218