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CD200 antibody (CD200) Internal Region Primary Antibody

CD200 Reactivity: Human ELISA, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (1 reference) Independent Validation (1 validation)
Catalog No. ABIN3187966
$358.66
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  • Target See all CD200 Antibodies
    CD200
    Binding Specificity
    • 15
    • 10
    • 10
    • 9
    • 9
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 83
    • 24
    • 16
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 61
    • 39
    • 15
    Rabbit
    Clonality
    • 58
    • 54
    Polyclonal
    Conjugate
    • 44
    • 14
    • 12
    • 11
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD200 antibody is un-conjugated
    Application
    • 53
    • 41
    • 38
    • 23
    • 19
    • 14
    • 13
    • 12
    • 9
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    CD200 Polyclonal Antibody detects endogenous levels of CD200 protein.
    Characteristics
    Rabbit Polyclonal to CD200.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Immunogen
    Synthesized peptide derived from the Internal region of human CD200 (aa 171-220)
    Isotype
    IgG
  • Application Notes
    WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000,
    Restrictions
    For Research Use only
  • Validation #103457 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    David A. Clark, Department of Medicine and Department of Pathology and Molecular Medicine, McMaster University
    No.
    #103457
    Date
    11/30/2018
    Antigen
    CD200
    Lot Number
    6839401
    Method validated
    Immunohistochemistry
    Positive Control

    Human 7 week elective termination placenta

    Negative Control

    KLH antibody ABIN401183

    Diluent

    Notes

    Passed. The CD200 antibody ABIN318966 specifically recognizes its antigen in human placental tissue.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3187966
    Secondary Antibody
    Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232)
    Full Protocol
    • Fix human placental tissue in 10% buffered formalin for 24h at RT (22-23°C), process, and embedded in paraffin.
    • Cut paraffin blocks with a Leica CM2255 Microtome into 4µm sections.
    • Affix sections to positively charged slides and air dry ON at RT.
    • Dewax the slides and hydrate on the automated Leica BOND Rx stainer.
    • Antigen retrieval on a Leica BOND RX automated stainer using epitope retrieval buffer 2 (Leica, AR9640, lot ER20172).
    • Stain slides with primary
      • rabbit anti- CD200 antibody (AA 45-95) (Antibodies-online, ABIN761396, lot 980502W),
      • rabbit anti-CD200 antibody (AA 170-220) (Antibodies-online, ABIN318966 lot 6839401) (AA 170-220), or
      • rabbit anti-KLH antibody (Antibodies-online, ABIN401183, lot 304770)
    • diluted 1:200 in Power Vision IHC Super Blocker (Leica, PV6122). The staining protocol incorporates a modified Leica standard protocol IHC-F (which omits the post-primary step) and uses the standard times outlined in the machine protocol.
    • Stain sections with Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232) containing peroxidase block, post primary antibody, polymer as well as DAB chromogen and hematoxylin counterstain for times outlined in the standard protocol IHC-F.
    • Remove slides from the Leica Bond Rx and then dehydrate in ethanol and clear in xylene.
    • Mount slides mounted in Fisher Chemical Permount Mounting Medium (Fisher Scientific, SP15-500, lot 162767).
    • Once the slide-coverslip edges are dry, scan the slides using Leica Imagescope (40x) and photograph into jpg files at 400x.
    Experimental Notes
    • Several dilutions of ABIN318966 were tested and optimal staining was obtained at a dilution as low as 1:200.

    • The staining pattern of ABIN318966 differs from our result using ABIN761396 which matched RB846 and observations made by others. The result may be explainable by ABIN318966 reacting with variants of CD200 which lack upstream exon 3 coded AA that are eliminated along with exon 2 coded AA by alternative splicing so as to produce the truncated form of CD200 (CD200tr). Removing exon 2 + some exon 3 coded AA may expose epitopes in the downstream exon 3 + exon 4 region of CD200tr that are detected by antibody to the 170-220 AA region of CD200 that are in exon 4 of CD200 variant 1.

    • ABIN761396 stained villus trophoblasts as described in Clark et al. (2017).

    • ABIN761396 stained villus cytotrophoblasts and syncytiotrophoblasts but ABIN3187966 did not show convincing staining.

  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C.
  • Clark, Reihani, Arredondo, Ask, Foster: "CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells." in: Journal of reproductive immunology, Vol. 150, pp. 103477, (2022) (PubMed).

  • Target
    CD200
    Alternative Name
    CD200 (CD200 Products)
    Synonyms
    MOX1, MOX2, MRC, OX-2, CD200, Cspmo2, MRCOX2, Mox2, DKFZp459C0716, OX2, CD200 molecule, Cd200 molecule, CD200 antigen, CD200, Cd200
    Molecular Weight
    31 kDa
    Gene ID
    4345
    UniProt
    P41217
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