CD7 antibody

Catalog No. ABIN120616

Product Details anti-CD7 Antibody

Target: CD7

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody reacts with CD7, a 40 kDa type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Precipitation methods and ion exchange chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human acute myelogenous leukaemia cell line KG-1

Clone: MEM-186

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Flow Cytometry. Western Blotting under non reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 5 m g/mL, Positive tissue: spleen
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD7

Target: CD7

Alternative Name: CD7 (CD7 Products)

Background: CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.Synonyms: GP40, Leu-9, T-cell leukemia antigen, TP41

Gene ID: 924

UniProt: P09564

Pathways: Cell-Cell Junction Organization