CD7 antibody

Catalog No. ABIN94218

Cited in 1 publication. The Mouse Monoclonal anti-CD7 antibody (Clone MEM-186) (ABIN94218) specifically detects CD7 in FACS, WB, IP and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD7 antibody (ABIN94218)

Target: CD7

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-186

Product Details anti-CD7 Antibody

Purpose: Anti-Hu CD7 Purified

Cross-Reactivity (Details): Human

Characteristics: The antibody MEM-186 reacts with an extracellular epitope of CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human acute myelogenous leukaemia cell line KG-1.

Isotype: IgG1, kappa

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: spleen.
Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD7 antibody (ABIN94218)

Rappl, Riet, Awerkiew, Schmidt, Hombach, Pfister, Abken: "The CD3-zeta chimeric antigen receptor overcomes TCR Hypo-responsiveness of human terminal late-stage T cells." in: PLoS ONE, Vol. 7, Issue 1, pp. e30713, (2012) (PubMed).

Target Details for CD7

Target: CD7

Alternative Name: CD7

Background: CD7 Molecule,CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.,GP40, TLA, TP41, LEU-9

Gene ID: 924

UniProt: P09564

Pathways: Cell-Cell Junction Organization