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CD99 antibody (Isoform CD99R)

CD99 Reactivity: Human FACS, WB, IP Host: Mouse Monoclonal MEM-131 unconjugated

Catalog No. ABIN120623

Datasheet as PDF

Target See all CD99 Antibodies

CD99
Binding Specificity
15

6

4

4

2

2

2

2

1

1

1

1

1

1

1
Isoform CD99R
Reactivity
100

24

9

6

1
Human
Host
73

47

4
Mouse
Compatible Secondaries
Clonality
69

55
Monoclonal
Conjugate
51

11

10

7

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD99 antibody is un-conjugated
Application
59

52

32

29

23

18

14

13

12

12

6

5

4

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Specificity

The antibody reacts with CD99R, an epitope restricted to a subset of CD99 Molecule expressed on myeloid cells, NK cells and T lymphocytes.

Purification

Precipitation methods

Purity

> 95 % (by SDS-PAGE)

Immunogen

HPB-ALL human peripheral blood leukemia T-cell line

Clone

MEM-131

Isotype

IgM

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CD99 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

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CD99 Reactivity: Human FACS, CyTOF Host: Mouse Monoclonal 3B2-TA8 unconjugated

anti-CD99 (CD99) (AA 27-161) antibody
CD99 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD99 (CD99) (AA 26-160) antibody
CD99 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD99 (CD99) antibody (PE)
CD99 Reactivity: Human FACS Host: Mouse Monoclonal 3B2-TA8 PE

anti-CD99 (CD99) antibody
CD99 Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 5C8 unconjugated

anti-CD99 (CD99) antibody
CD99 Reactivity: Human FACS, WB, IHC (p), IP Host: Mouse Monoclonal MEM-131 unconjugated

Application Notes

Flow Cytometry. Immunoprecipitation. Western Blotting under non reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 m g/mL, Positive tissue: thymic lymphocytes
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store the antibody at 2 - 8 °C. Do not freeze.
Shelf Life: one year from despatch.

Expiry Date

12 months
Target

CD99

Alternative Name

CD99 / MIC2 (CD99 Products)

Target Type

Viral Protein

Background

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.Synonyms: 12E7, E2 antigen, MIC2X, MIC2Y, Protein MIC2, T-cell surface glycoprotein E2

Gene ID

4267

UniProt

P14209