CD99 antibody (PE)

Catalog No. ABIN1027670

Product Details anti-CD99 Antibody

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Microcytotoxicity Assay (MCA)

Specificity: The mouse monoclonal antibody 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.
HLDA VI., WS Code T 6T-097, BP 534

Cross-Reactivity (Details): Human

Immunogen: Human thymocytes

Clone: 3B2-TA8

Isotype: IgG2a

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD99 antibody (ABIN1027670)

Brémond, Meynet, Mahiddine, Coito, Tichet, Scotlandi, Breittmayer, Gounon, Gleeson, Bernard, Bernard: "Regulation of HLA class I surface expression requires CD99 and p230/golgin-245 interaction." in: Blood, Vol. 113, Issue 2, pp. 347-57, (2009) (PubMed).

Kueng, Leb, Haiderer, Raposo, Thery, Derdak, Schmetterer, Neunkirchner, Sillaber, Seed, Pickl: "General strategy for decoration of enveloped viruses with functionally active lipid-modified cytokines." in: Journal of virology, Vol. 81, Issue 16, pp. 8666-76, (2007) (PubMed).

Target Details for CD99

Target: CD99

Alternative Name: CD99 (CD99 Products)

Synonyms: 1110061M03Rik antibody, 2410026K10Rik antibody, D4 antibody, Pilr-l antibody, pilr-1 antibody, HBA71 antibody, MIC2 antibody, MIC2X antibody, MIC2Y antibody, MSK5X antibody, CD99 antigen antibody, CD99 molecule (Xg blood group) antibody, CD99 molecule antibody, Cd99 antibody, CD99 antibody

Target Type: Viral Protein

Background: CD99 is a ubiquitous transmembrane type I sialoglycoprotein of a unique and poorly characterized protein family. CD99 is heavily O-glycosylated and was described as a T cell costimulator and strong activator of integrin-mediated actin cytoskeleton assembly, promoting cell adhesion and homotypic aggregation, immediate arrest on an inflamed vascular endothelium, and cell migration through it. Ligation of CD99 under some conditions can lead to apoptosis. Originally CD99 was described as a human thymus leukemia antigen, an Ewing´s sarcoma-specific membrane marker, and an adhesion molecule involved in spontaneous rosette formation of T cells with erythrocytes.