Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1) antibody

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Antigen
Synonyms EBP-50, NHE-RF, NHERF-1, NHERF1, EBP50, NHERF, NPHLOP2
Reactivity
Human
(79), (19), (18)
Host
Mouse
(55), (26), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(71), (27), (18), (18), (16), (11), (9), (7), (3), (2), (1), (1)
Pubmed 1 reference available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Bacterially produced recombinant full-length human NHERF
Clone EBP10
Isotype IgG2b
Specificity The antibody reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
Purification Affinity Chromatography on Protein A
Alternative Name SLC9A3R1 / NHERF (SLC9A3R1 Antibody Abstract)
Background NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.Synonyms: EBP50, Ezrin-radixin-moesin-binding phosphoprotein 50, NHERF-1, NPHLOP2, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1
Gene ID 9368
UniProt O14745
Application Notes Immunoprecipitation. Western Blotting: 2 m g/mL, 60 min on vertical incubator. Positive control: RAJI human lymphoma cell lysate. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. 10 % separating SDS-PAGE gel. Immunohistochemistry (paraffin sections): Recommended dilution: 5 m g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Background publications Brdicková, Brdicka, Andera et al.: "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton." in: FEBS letters, Vol. 507, Issue 2, pp. 133-6, 2001 (PubMed).

Catalog No. ABIN120629
$308.00
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