Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1) antibody

Details for Product No. ABIN94437
Antigen
Reactivity
Human
104
53
46
3
3
3
2
1
1
1
1
1
Host
Mouse
83
21
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
78
27
20
20
18
16
13
4
1
1
1
1
Options
Immunogen Bacterially produced recombinant full-length human NHERF1.
Clone EBP-10
Isotype IgG2b
Specificity The antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
Cross-Reactivity (Details) Other not tested, Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name NHERF1 / EBP50 (SLC9A3R1 Antibody Abstract)
Background NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
Pathways Proton Transport, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, SARS-CoV-2 Protein Interactome
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.