IL-2 antibody

Catalog No. ABIN1383940

Product Details anti-IL-2 Antibody

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Functional Studies (Func), ELISpot

Specificity: Recognises both natural and recombinant human IL-2

Purification: Ion exchange chromatography

Sterility: 0.22 μm filtered

Immunogen: Natural human IL-2,Myeloma X63/AG.8653 x Balb/c spleen cells

Clone: B-G5

Isotype: IgG1 kappa

Biological Activity Comment: Inhibits IL-2 induced proliferation on Kit 225 cell line

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store at +2-8°C for 12 months. DO NOT FREEZE.

References for anti-IL-2 antibody (ABIN1383940)

Koopman, Mortier, Hofman, Mathy, Koutsoukos, Ertl, Overend, van Wely, Thomsen, Wahren, Voss, Heeney: "Immune-response profiles induced by human immunodeficiency virus type 1 vaccine DNA, protein or mixed-modality immunization: increased protection from pathogenic simian-human immunodeficiency virus viraemia with protein/DNA combination." in: The Journal of general virology, Vol. 89, Issue Pt 2, pp. 540-53, (2008) (PubMed).

Kobari, Giarratana, Gluckman, Douay, Rosenzwajg: "Ex vivo expansion does not alter the capacity of umbilical cord blood CD34+ cells to generate functional T lymphocytes and dendritic cells." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 9, pp. 2150-7, (2006) (PubMed).

Koopman, Mortier, Hofman, Niphuis, Fagrouch, Norley, Sutter, Liljeström, Heeney: "Vaccine protection from CD4+ T-cell loss caused by simian immunodeficiency virus (SIV) mac251 is afforded by sequential immunization with three unrelated vaccine vectors encoding multiple SIV antigens." in: The Journal of general virology, Vol. 85, Issue Pt 10, pp. 2915-24, (2004) (PubMed).

Van Rhijn, Van den Berg, Ang, Admiraal, Logtenberg: "Expansion of human gammadelta T cells after in vitro stimulation with Campylobacter jejuni." in: International immunology, Vol. 15, Issue 3, pp. 373-82, (2003) (PubMed).

Frétier, Besse, Delwail, Garcia, Morel, Leprivey-Lorgeot, Wijdenes, Praloran, Lecron: "Cyclosporin A inhibition of macrophage colony-stimulating factor (M-CSF) production by activated human T lymphocytes." in: Journal of leukocyte biology, Vol. 71, Issue 2, pp. 289-94, (2002) (PubMed).

Muñoz, van Seventer, Semnani, Leff: "Augmentation of LTC(4) synthesis in human eosinophils caused by CD3-stimulated Th2-like cells in vitro." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 278, Issue 6, pp. L1172-9, (2000) (PubMed).

Groux, Bigler, de Vries, Roncarolo: "Inhibitory and stimulatory effects of IL-10 on human CD8+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3188-93, (1998) (PubMed).

Peng, Remacle, Kasran, Huylebroeck, Ceuppens: "IL-12 up-regulates CD40 ligand (CD154) expression on human T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1166-72, (1998) (PubMed).

Palmer, van Seventer: "Human T helper cell differentiation is regulated by the combined action of cytokines and accessory cell-dependent costimulatory signals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 6, pp. 2654-62, (1997) (PubMed).

Pilson, Levin, Desai, Reik, Lin, Korkmaz-Duffy, Campbell, Tso, Kerwin, Hakimi: "Bispecific humanized anti-IL-2 receptor alpha beta antibodies inhibitory for both IL-2- and IL-15-mediated proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 3, pp. 1543-56, (1997) (PubMed).

Fisfalen, Palmer, Van Seventer, Soltani, Sawai, Kaplan, Hidaka, Ober, DeGroot: "Thyrotropin-receptor and thyroid peroxidase-specific T cell clones and their cytokine profile in autoimmune thyroid disease." in: The Journal of clinical endocrinology and metabolism, Vol. 82, Issue 11, pp. 3655-63, (1997) (PubMed).

Tortorella, Schulze-Koops, Thomas, Splawski, Davis, Picker, Lipsky: "Expression of CD45RB and CD27 identifies subsets of CD4+ memory T cells with different capacities to induce B cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 149-62, (1995) (PubMed).

Picker, Singh, Zdraveski, Treer, Waldrop, Bergstresser, Maino: "Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry." in: Blood, Vol. 86, Issue 4, pp. 1408-19, (1995) (PubMed).

Kuiper, de Jong, Brouwer, Lammers, Wijdenes, van Lier: "Influence of CD28 co-stimulation on cytokine production is mainly regulated via interleukin-2." in: Immunology, Vol. 83, Issue 1, pp. 38-44, (1995) (PubMed).

de Jong, Brouwer, Kuiper, Hooibrink, Miedema, van Lier: "Maturation- and differentiation-dependent responsiveness of human CD4+ T helper subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 8, pp. 2795-802, (1992) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2 (IL2 Products)

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation