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IL-2 antibody (PE)

Name: IL-2 antibody (PE)
Brand: antibodies-online
SKU: ABIN7505991
Price: 484.24 USD
Availability: InStock

IL2 Reactivity: Human FACS Host: Mouse Monoclonal 35C3 PE

Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (IL-2 antibody (PE))

FACS

Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue antibody (4 μL reagent / 100 μL of peripheral whole blood) and intracellular staining using anti-human IL-2 (35C3) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (IL-2 antibody (PE))

FACS

Separation of human IL-2 positive CD3 positive lymphocytes (red-filled) from IL-2 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (IL-2 antibody (PE))

FACS

3 images

Catalog No. ABIN7505991

Datasheet as PDF

Target See all IL-2 (IL2) Antibodies

IL-2 (IL2) (Interleukin 2 (IL2))
Reactivity
127

89

41

14

9

8

6

6

5

4

4

4

3

2

1

1

1

1
Human
Host
110

78

59

10

4

2

1
Mouse
Compatible Secondaries
Clonality
137

123

1
Monoclonal
Conjugate
144

37

23

21

14

9

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This IL-2 antibody is conjugated to PE
Application
155

122

68

63

40

28

24

22

14

14

13

13

13

11

11

8

6

5

4

3

3

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Purpose

Anti-Hu IL-2 PE

Specificity

The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2, secreted or intracellular).

Purification

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

recombinant human IL-2

Clone

35C3

Isotype

IgG2b kappa

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IL2 Reactivity: Human WB, ELISA, FACS Host: Rabbit Monoclonal unconjugated

Application Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions

For Research Use only
Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Target

IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name

IL-2 (IL2 Products)

Synonyms

IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody

Background

Interleukin 2,IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.,Interleukin 2

Gene ID

3558

UniProt

P60568

Pathways

JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation