Transferrin Receptor antibody

Catalog No. ABIN181740

Product Details anti-Transferrin Receptor Antibody

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: This antibody recognises murine CD71, a 200 kDa antigen which is otherwise known as transferrin receptor.

Purification: Affinity chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Balb/c macrophage precursor cell hybrids. Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.

Clone: ER-MP21

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation. Functional Assays. Flow Cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cellsin 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / TFRC (TFRC Products)

Background: CD71 is expressed by all proliferating cell populations and exists as a homodimer at the cell surface. Clone ER-MP21 inhibits iron uptake and cell proliferation but does not compete with transferrin for binding to its receptor, which suggests that clone ER-MP21 binds to an epitope that is different from the transferrin-binding site.Synonyms: TfR1, Transferrin receptor protein 1, p90

Gene ID: 22042

NCBI Accession: NP_035768

UniProt: Q62351

Pathways: Transition Metal Ion Homeostasis