RuvB-Like 2 (E. Coli) (RUVBL2) (N-Term) antibody

Details for Product No. ABIN182548
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Antigen
Synonyms MGC52995, rvb2, ecp51, tip48, cgi-46, reptin, tip49b, xReptin, MGC69398, RUVBL2, ECP51, INO80J, REPTIN, RVB2, TIH2, TIP48, TIP49B, wu:fi25f01, zreptin, mp47, p47
Epitope
N-Term
(8), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Zebrafish (Danio rerio), Xenopus laevis, Human
(37), (9), (8), (4), (2), (2), (1), (1)
Host
Rabbit
(29), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(33), (12), (12), (10), (8), (4), (4), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN182548
229.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human RUVBL2
Sequence IERIGAHSHI RGLGLDDALE PRQASQGMVG QLAARRAAGV VLEMIREGKI
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 90 %, Zebrafish : 90 %
Characteristics This is a rabbit polyclonal antibody against RUVBL2. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name RUVBL2
Background RuvB-Like 2 (48-kDa TATA box-binding protein-interacting protein, Reptin 52, RUVBL2) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this protein has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.
Molecular Weight 51 kDa
Gene ID 10856
NCBI Accession NP_006657, NM_006666
UniProt Q9Y230
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 463 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-RuvB-Like 2 (E. Coli) (RUVBL2) (N-Term) antibody WB Suggested Anti-RUVBL2 Antibody Titration: 2.0-4.0ug/ml
Positive Control: Daudi cell lysate
anti-RuvB-Like 2 (E. Coli) (RUVBL2) (N-Term) antibody (2) anti-RuvB-Like 2 (E. Coli) (RUVBL2) (N-Term) antibody (Image 2)
anti-RuvB-Like 2 (E. Coli) (RUVBL2) (N-Term) antibody (3) anti-RuvB-Like 2 (E. Coli) (RUVBL2) (N-Term) antibody (Image 3)
anti-RuvB-Like 2 (E. Coli) (RUVBL2) (N-Term) antibody (4) Sample Type: Lane 1:241 µg untransfected H1299 cells Lane 2: 041 µg siRUVBL2 transfected H1299 cells
Primary Antibody Dilution: 1:0000
Secondary Antibody: Anti-rabbit-HRP
Secondary Antibody Dilution: 1:0000 Color/Signal Descriptions: RUVBL2
Gene Name: Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey.

Submitted by:
anti-RuvB-Like 2 (E. Coli) (RUVBL2) (N-Term) antibody (5) Human urinary bladder
Background publications Bauer, Chauvet, Huber et al.: "Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity." in: The EMBO journal, Vol. 19, Issue 22, pp. 6121-30, 2000 (PubMed).

Validation Images
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