Mediator Complex Subunit 17 (MED17) (N-Term) antibody

Details for Product No. ABIN182953
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Antigen
Synonyms crsp6, CRSP6, CRSP77, DRIP80, TRAP80, 77kDa, C330002H14Rik, Crsp6, Trap80
Epitope
N-Term
(9), (8), (8), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus), Dog (Canine), Mouse (Murine), Cow (Bovine), Xenopus laevis, Human, Chicken
(56), (26), (14), (12), (12), (12), (12)
Host
Rabbit
(41), (16)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
(44), (29), (10), (9), (7), (7), (6), (2)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN182953
289.00 $
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  • +1 404 474 4654
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Immunogen Synthetic peptide directed towards the N terminal of human CRSP6
Sequence AAQILLKGAE RLTKSVTENQ ENKLQRDFNS ELLRLRQHWK LRKVGDKILG
Predicted Reactivity Rat :100 %,   Dog :100 %,   Bovine :100 %,   Human :100 %,   Mouse :90 %,   African clawed frog :80 %,   Western clawed frog :80 %,   Chicken :80 %,  
Characteristics This is a rabbit polyclonal antibody against CRSP6. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name CRSP6
Background The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by CRSP6 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Molecular Weight 73 kDa
Gene ID 9440
NCBI Accession NM_004268, NP_004259
UniProt Q9NVC6
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 651 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Mediator Complex Subunit 17 (MED17) (N-Term) antibody WB Suggested Anti-CRSP6 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysate
anti-Mediator Complex Subunit 17 (MED17) (N-Term) antibody (2) anti-Mediator Complex Subunit 17 (MED17) (N-Term) antibody (Image 2)
anti-Mediator Complex Subunit 17 (MED17) (N-Term) antibody (3) Rabbit Anti-MED17 Antibody ,Paraffin Embedded Tissue: Human neural cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 µg/mL
Magnification:.00X
anti-Mediator Complex Subunit 17 (MED17) (N-Term) antibody (4) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Background publications Wang, Sharma, Ren et al.: "A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 42852-8, 2002 (PubMed).

Validation Images
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