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Endothelial Differentiation Related Factor 1 (EDF1) (N-Term) antibody

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(6), (5), (5), (4), (3), (1), (1), (1), (1), (1)
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(45), (13), (13), (4), (4), (3), (2), (2), (1), (1)
(25), (15), (6)
(1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(39), (19), (11), (8), (5), (2)
Pubmed 2 references available
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Quantity 100 μg
Shipping to United States ( )
Immunogen Synthetic peptide directed towards the N terminal of human EDF1
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against EDF1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name EDF1 (EDF1 Antibody Abstract)
Background EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.
Molecular Weight 16 kDa
Gene ID 8721
NCBI Accession NP_003783, NM_003792
UniProt O60869
Research Area Signaling, Metabolism, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 148 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Endothelial Differentiation Related Factor 1 (EDF1) (N-Term) antibody (ABIN183557) WB Suggested Anti-EDF1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:...
Background publications Brendel, Schoonjans, Botrugno et al.: "The small heterodimer partner interacts with the liver X receptor alpha and represses its transcriptional activity." in: Molecular endocrinology (Baltimore, Md.), Vol. 16, Issue 9, pp. 2065-76, 2002 (PubMed).

Brendel, Gelman, Auwerx: "Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism." in: Molecular endocrinology (Baltimore, Md.), Vol. 16, Issue 6, pp. 1367-77, 2002 (PubMed).

Catalog No. ABIN183557

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