Endothelial Differentiation Related Factor 1 (EDF1) (N-Term) antibody

Details for Product No. ABIN183557
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Synonyms edf-1, mbf1, EDF-1, MBF1, 0610008L11Rik, AA409425, CAP-19, zgc:73192, zgc:86829
(4), (4), (4), (4), (1), (1), (1), (1)
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Xenopus laevis, Pig (Porcine), Human, Chicken
(32), (6), (6), (2), (2), (2), (1), (1), (1)
(14), (13), (5)
(1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(28), (16), (9), (7), (5), (2), (1), (1)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN183557
229.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human EDF1
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against EDF1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name EDF1
Background EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.
Molecular Weight 16 kDa
Gene ID 8721
NCBI Accession NP_003783, NM_003792
UniProt O60869
Research Area Signaling, Metabolism, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 148 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Endothelial Differentiation Related Factor 1 (EDF1) (N-Term) antibody WB Suggested Anti-EDF1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate
Background publications Brendel, Gelman, Auwerx: "Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism." in: Molecular endocrinology (Baltimore, Md.), Vol. 16, Issue 6, pp. 1367-77, 2002 (PubMed).

General Brendel, Schoonjans, Botrugno et al.: "The small heterodimer partner interacts with the liver X receptor alpha and represses its transcriptional activity." in: Molecular endocrinology (Baltimore, Md.), Vol. 16, Issue 9, pp. 2065-76, 2002 (PubMed).

Validation Images
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