EDF1 antibody (Middle Region)

Catalog No. ABIN2780547

The Rabbit Polyclonal anti-EDF1 antibody (ABIN2780547) specifically detects EDF1 in WB, IHC and IF. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig and Horse samples.

Quick Overview for EDF1 antibody (Middle Region) (ABIN2780547)

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EDF1 Antibody

Binding Specificity: Middle Region

Purpose: EDF1 Antibody - middle region

Sequence: INEKPQVIAD YESGRAIPNN QVLGKIERAI GLKLRGKDIG KPIEKGPRAK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EDF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EDF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38383-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EDF1

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Alternative Name: EDF1

Background:

Background Information: EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene. This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.

Gene Name: Endothelial differentiation-related factor 1

Alternative Symbols: MBF1, EDF-1, CFAP280

Protein Name: Endothelial differentiation-related factor 1

Molecular Weight: 16kDa

Gene ID: 8721

NCBI Accession: NP_003783

UniProt: O60869