EDF1 antibody (N-Term)

Catalog No. ABIN2776405

Product Details anti-EDF1 Antibody

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Sequence: MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against EDF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EDF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 148 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EDF1 antibody (ABIN2776405)

Leidi, Mariotti, Maier: "The effects of silencing EDF-1 in human endothelial cells." in: Atherosclerosis, Vol. 211, Issue 1, pp. 55-60, (2010) (PubMed).

Leidi, Mariotti, Maier: "EDF-1 contributes to the regulation of nitric oxide release in VEGF-treated human endothelial cells." in: European journal of cell biology, Vol. 89, Issue 9, pp. 654-60, (2010) (PubMed).

Leidi, Mariotti, Maier: "Transcriptional coactivator EDF-1 is required for PPARgamma-stimulated adipogenesis." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 16, pp. 2733-42, (2009) (PubMed).

Target Details for EDF1

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Alternative Name: EDF1 (EDF1 Products)

Synonyms: edf-1 antibody, mbf1 antibody, EDF-1 antibody, MBF1 antibody, 0610008L11Rik antibody, AA409425 antibody, CAP-19 antibody, zgc:73192 antibody, zgc:86829 antibody, endothelial differentiation related factor 1 S homeolog antibody, endothelial differentiation related factor 1 antibody, endothelial differentiation-related factor 1 antibody, Mbf1p antibody, edf1.S antibody, EDF1 antibody, Edf1 antibody, edf1 antibody, MBF1 antibody

Background: EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the tran
Alias Symbols: EDF-1, MBF1, MGC9058
Protein Interaction Partner: MEOX2, UBC, IDH3G, UBE2I, MAPK6, NRL, JUN, CEBPA, NR1H3, NR5A2, PPARG, TBP, TAF1, NR5A1, RXRA, PRKCA, ATF2, CREB1, ATF1, CALM3, CALM1,
Protein Size: 148

Molecular Weight: 16 kDa

Gene ID: 8721

NCBI Accession: NM_003792, NP_003783

UniProt: O60869