EDF1 antibody (N-Term)

Catalog No. ABIN2776405

There are 3+ publications for this product available. The Rabbit Polyclonal anti-EDF1 antibody is suitable to detect EDF1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB and IF.

Quick Overview for EDF1 antibody (N-Term) (ABIN2776405)

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-EDF1 Antibody

Binding Specificity: N-Term

Purpose: EDF1 Antibody - N-terminal region

Sequence: MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against EDF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EDF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP35695-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EDF1 antibody (ABIN2776405)

Leidi, Mariotti, Maier: "The effects of silencing EDF-1 in human endothelial cells." in: Atherosclerosis, Vol. 211, Issue 1, pp. 55-60, (2010) (PubMed).

Leidi, Mariotti, Maier: "EDF-1 contributes to the regulation of nitric oxide release in VEGF-treated human endothelial cells." in: European journal of cell biology, Vol. 89, Issue 9, pp. 654-60, (2010) (PubMed).

Leidi, Mariotti, Maier: "Transcriptional coactivator EDF-1 is required for PPARgamma-stimulated adipogenesis." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 16, pp. 2733-42, (2009) (PubMed).

Target Details for EDF1

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Alternative Name: EDF1

Background:

Background Information: EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the tran

Gene Name: Endothelial differentiation-related factor 1

Alternative Symbols: MBF1, EDF-1, CFAP280

Protein Name: Endothelial differentiation-related factor 1

Molecular Weight: 16kDa

Gene ID: 8721

NCBI Accession: NP_003783

UniProt: O60869