Non-POU Domain Containing, Octamer-Binding (NONO) (N-Term) antibody

Details for Product No. ABIN184023
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Antigen
Synonyms NMT55, NRB54, P54, P54NRB, nmt55, nrb54, p54nrb, xp54nrb, AA407051, AV149256, nonA
Epitope
N-Term
(10), (8), (7), (3), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Human, Chicken
(46), (14), (12), (7), (2), (2), (1), (1), (1)
Host
Rabbit
(33), (9), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(48), (21), (20), (14), (11), (6), (5), (4), (1)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN184023
229.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human NONO
Sequence KQNHTPRKHH QHHHQQQHHQ QQQQQPPPPP IPANGQQASS QNEGLTIDLK
Predicted Reactivity Horse : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Bovine : 90 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against NONO. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name NONO
Background NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
Molecular Weight 52 kDa
Gene ID 4841
NCBI Accession NM_007363, NP_031389
UniProt Q15233
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 471 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (N-Term) antibody WB Suggested Anti-NONO Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (N-Term) antibody (2) anti-Non-POU Domain Containing, Octamer-Binding (NONO) (N-Term) antibody (Image 2)
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (N-Term) antibody (3) Sample Type: subnuclear bodies-paraspeckles
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (N-Term) antibody (4) WB Suggested Anti-NONO
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate
Product cited in: Lazrek, Goffard, Schanen et al.: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006 (PubMed).

Background publications Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Validation Images
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