NONO antibody (N-Term)

Catalog No. ABIN2779027

Cited in 2+ publications. The Rabbit Polyclonal anti-NONO antibody (ABIN2779027) specifically detects NONO in WB and IF. The antibody is reactive with Human, Mouse, Rat, Dog, Horse, Cow and Guinea Pig samples.

Quick Overview for NONO antibody (N-Term) (ABIN2779027)

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NONO antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NONO Antibody

Binding Specificity: N-Term

Purpose: NONO Antibody - N-terminal region

Sequence: KQNHTPRKHH QHHHQQQHHQ QQQQQPPPPP IPANGQQASS QNEGLTIDLK

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NONO. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NONO

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106608

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NONO antibody (ABIN2779027)

Bubenik, Ladd, Gerber, Budiman, Driscoll: "Known turnover and translation regulatory RNA-binding proteins interact with the 3' UTR of SECIS-binding protein 2." in: RNA biology, Vol. 6, Issue 1, pp. 73-83, (2009) (PubMed).

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Alternative Name: NONO

Background:

Background Information: NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.

Gene Name: Non-POU domain containing, octamer-binding

Alternative Symbols: P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114

Protein Name: Non-POU domain-containing octamer-binding protein

Molecular Weight: 52kDa

Gene ID: 4841

NCBI Accession: NP_031389

UniProt: Q15233