Prostaglandin-Endoperoxide Synthase 1 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS1) (C-Term) antibody

Details for Product No. ABIN184671
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Antigen
Synonyms COX-1, cox-1, PTGS1, Ptgs1, COX1, Cox-1, Cox-3, PGHS-1, PHS 1, Pghs1, Cox1, Cox3, cox1, zCOX-1, COX3, PCOX1, PES-1, PGG/HS, PGHS1, PHS1, PTGHS, COX-3
Epitope
C-Term
(17), (10), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(83), (36), (27), (22), (14), (14), (11), (2), (2), (1), (1), (1), (1)
Host
Goat
(69), (22), (15)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(73), (37), (34), (15), (10), (10), (9), (5), (5), (4), (2), (2)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184671
374.27 $
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Immunogen Peptide with sequence C-QDDGPAVERPSTEL, from the C Terminus of the protein sequence according to NP_000953.2, NP_542158.1.
Specificity This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name COX1 / PTGS1
Background Synonyms: PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase, RP11-542K23.6
Gene ID 5742
NCBI Accession NP_542158, NP_000953
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60 kDa and 70 kDa bands observed in lysates of cell line HeLa (calculated MW of 64.5 kDa according to NP_542158.1 and 68.7 kDa according to NP_000953.2) Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Heart shows patchy staining of myocardial fibres in longitudinal section. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Prostaglandin-Endoperoxide Synthase 1 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS1) (C-Term) antibody ABIN184671 staining (1µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Yokoyama, Tanabe: "Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme." in: Biochemical and biophysical research communications, Vol. 165, Issue 2, pp. 888-94, 1990 (PubMed).

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