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Prostaglandin-Endoperoxide Synthase 1 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS1) (C-Term) antibody

Details for Product No. ABIN184671
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Synonyms COX-1, cox-1, PTGS1, Ptgs1, COX1, Cox-1, Cox-3, PGHS-1, PHS 1, Pghs1, Cox1, Cox3, cox1, zCOX-1, COX3, PCOX1, PES-1, PGG/HS, PGHS1, PHS1, PTGHS, COX-3
(16), (10), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(80), (34), (27), (22), (14), (14), (12), (2), (2), (1), (1), (1), (1)
(69), (23), (14)
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(73), (37), (33), (15), (10), (10), (9), (5), (5), (4), (2), (2)
Pubmed 1 reference available
Quantity 100 µg
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Availability Available
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Catalog No. ABIN184671
374.27 $
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Immunogen Peptide with sequence C-QDDGPAVERPSTEL, from the C Terminus of the protein sequence according to NP_000953.2, NP_542158.1.
Specificity This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name COX1 / PTGS1
Background Synonyms: PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase, RP11-542K23.6
Gene ID 5742
NCBI Accession NP_000953.2, NP_542158.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60 kDa and 70 kDa bands observed in lysates of cell line HeLa (calculated MW of 64.5 kDa according to NP_542158.1 and 68.7 kDa according to NP_000953.2) Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Heart shows patchy staining of myocardial fibres in longitudinal section. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Prostaglandin-Endoperoxide Synthase 1 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS1) (C-Term) antibody ABIN184671 staining (1µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Yokoyama, Tanabe: "Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme." in: Biochemical and biophysical research communications, Vol. 165, Issue 2, pp. 888-94, 1990 (PubMed).

Validation Images
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