PTGS1 antibody (AA 318-599)

Catalog No. ABIN3042528

Cited in 3+ publications. The Rabbit Polyclonal anti-PTGS1 antibody (ABIN3042528) specifically detects PTGS1 in WB, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PTGS1 antibody (AA 318-599) (ABIN3042528)

Target: PTGS1 (Prostaglandin-Endoperoxide Synthase 1 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTGS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PTGS1 Antibody

Binding Specificity: AA 318-599

Purpose: Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (ABIN3042528). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human COX1 recombinant protein (Position: E318-L599). Human COX1 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat COX1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-PTGS1 antibody (ABIN3042528)

Ahmadieh, Nourinia, Hafezi-Moghadam, Sabbaghi, Nakao, Zandi, Yaseri, Tofighi, Akbarian: "Intravitreal injection of a Rho-kinase inhibitor (fasudil) combined with bevacizumab versus bevacizumab monotherapy for diabetic macular oedema: a pilot randomised clinical trial." in: The British journal of ophthalmology, (2018) (PubMed).

Zhen, Zong, Gao, Cao, Jiang, Chen, Wang, Sun, Peng, Bai, Li: "Preparation and characterization of a novel aspirin derivative with anti-thrombotic and gastric mucosal protection properties." in: PLoS ONE, Vol. 9, Issue 6, pp. e98513, (2015) (PubMed).

Duan, Que, Lv, Li, Yin, Zhang: "Tolerance of neurite outgrowth to Rho kinase inhibitors decreased by cyclooxygenase-2 inhibitor." in: Neural regeneration research, Vol. 7, Issue 34, pp. 2705-12, (2014) (PubMed).

Target Details for PTGS1

Target: PTGS1 (Prostaglandin-Endoperoxide Synthase 1 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS1))

Alternative Name: PTGS1

Background:

Synonyms: Prostaglandin G/H synthase 1,1.14.99.1,Cyclooxygenase-1,COX-1,Prostaglandin H2 synthase 1,PGH synthase 1,PGHS-1,PHS 1,Prostaglandin-endoperoxide synthase 1,PTGS1,COX1,

Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma.

Background: Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1) or mitochondrial cytochrome c oxidase subunit 1, is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, it was demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91 % amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

Sequence Similarities: Belongs to the prostaglandin G/H synthase family.

Molecular Weight: 65-72 kDa

UniProt: P23219