Thioredoxin Reductase 1 (TXNRD1) (C-Term) antibody

Details for Product No. ABIN184781
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Antigen
Synonyms TrxR1, cb682, fb83a08, wu:fb83a08, GB14972, MGC85342, NADPH-dependent thioredoxin reductase C, T32G6.20, T32G6_20, GRIM-12, TR, TR1, TRXR1, TXNR, Tr
Epitope
C-Term
(4), (4), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(58), (17), (17), (16), (13), (13)
Host
Goat
(34), (18), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(39), (30), (15), (15), (10), (7), (7), (7), (3), (2)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184781
374.27 $
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Immunogen Peptide with sequence TKRSGASILQAGC, from the C Terminus of the protein sequence according to NP_003321.2, NP_877393.1, NP_877419.1, NP_877420.1, NP_001087240.1.
Specificity This antibody is expected to recognise reported isoform 1 (NP_003321.2), isoform 2 (NP_877393.1, NP_877419.1, NP_877420.1) and isoform 3 (NP_001087240.1)
Cross-Reactivity Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Thioredoxin Reductase 1
Background Synonyms: TXNRD1, thioredoxin reductase 1, TR1, TXNR, GRIM-12, MGC9145, TR, TRXR1, KM-102-derived reductase-like factor, oxidoreductase, thioredoxin reductase GRIM-12
Gene ID 7296
NCBI Accession NP_001087240, NP_877420, NP_877393, NP_877419, NP_003321
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60 kDa band observed in Human Placenta lysates (predicted MW of 57 kDa according to NP_003321). Recommended for use at 0.1-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Thioredoxin Reductase 1 (TXNRD1) (C-Term) antibody ABIN184781 staining (0.1 ug/ml) of Human Placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Gasdaska, Gasdaska, Cochran et al.: "Cloning and sequencing of a human thioredoxin reductase." in: FEBS letters, Vol. 373, Issue 1, pp. 5-9, 1995 (PubMed).

Validation Images
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