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CARD11 antibody (Caspase Recruitment Domain Family, Member 11) (C-Term)

Details for Product anti-CARD11 Antibody No. ABIN184957, Supplier: Log in to see
Antigen
  • CARD11
  • LOC100232755
  • BENTA
  • BIMP3
  • CARMA1
  • PPBL
  • 0610008L17Rik
  • 2410011D02Rik
Alternatives
anti-Human CARD11 antibody for ELISA
Epitope
C-Term
27
7
6
4
3
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
67
29
5
4
4
1
1
Host
Goat
51
18
1
1
Clonality
Polyclonal
Conjugate
This CARD11 antibody is un-conjugated
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
64
37
32
16
6
5
4
3
2
2
Supplier
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Immunogen Peptide with sequence C-QRKTIWVDEDQL, from the C Terminus of the protein sequence according to NP_115791.3.
Blocking Peptide Blocking peptide for this product available: ABIN369694
Cross-Reactivity Mouse (Murine), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CARD11 (CARD11 Antibody Abstract)
Background Synonyms: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3
Gene ID 84433, 108723
NCBI Accession NP_115791
Research Area Cancer, Apoptosis/Necrosis, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150+65 kDa bands observed in lysates of cell line Jurkat (calculated MW of 133 kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-CARD11 antibody (Caspase Recruitment Domain Family, Member 11) (C-Term) (ABIN184957) ABIN184957 staining (1µg/ml) of human Jurkat cells (RIPA buffer, 35µg total protein p...
Background publications Bertin, Wang, Guo et al.: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001 (PubMed).