Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody

Details for Product No. ABIN184957
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Antigen
Synonyms BENTA, BIMP3, CARMA1, PPBL, 0610008L17Rik, 2410011D02Rik, CARD11, LOC100232755
Epitope
C-Term
(13), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(53), (36), (14), (14), (13), (12)
Host
Goat
(51), (6), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(44), (27), (19), (13), (10), (3), (2), (2), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN184957
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-QRKTIWVDEDQL, from the C Terminus of the protein sequence according to NP_115791.3.
Cross-Reactivity Mouse (Murine), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CARD11
Background Synonyms: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3
Gene ID 84433, 108723
NCBI Accession NP_115791.3
Research Area Cancer, Apoptosis/Necrosis, Adaptive Immunity
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150+65 kDa bands observed in lysates of cell line Jurkat (calculated MW of 133 kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody ABIN184957 staining (1µg/ml) of human Jurkat cells (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Bertin, Wang, Guo et al.: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001 (PubMed).

Hosts (51), (6), (1)
Reactivities (53), (36), (14), (14), (13), (12)
Applications (44), (27), (19), (13), (10), (3), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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