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Details for Product No. ABIN185021

Forkhead Box A1 (FOXA1) (C-Term) antibody

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Antigen
Synonyms
HNF3A, TCF3A, Hnf3a, Tcf3a, Hnf-3a, Tcf-3a, fkd7, forkhead-7, HNF-3a, HNF3-alpha, HNF3alpha, fkh2, foxa1, hnf3a, tFoxA1, tcf3a, FoxA4a, XFD-1, fkh1, pintallavis, xfkh1, xkfh-1, xfkh2, fkh, FOXA1, Ci-f ... show more
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
Clonality Polyclonal
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN185021
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Availability Ships within 5 Business Days
Immunogen Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
Cross-Reactivity Rat (Rattus), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name FOXA1 / HNF3A
Background Synonyms: FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha
Gene ID 3169, 15375, 25098
NCBI Accession NP_004487.2
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 50 kDa band observed in Human lysates of cell line HepG2 (calculated MW of 49.1 kDa according to NP_004487.2). Recommended concentration: 0.2-0.6 µg/mL. Additional bands of unknown identity were also consistently observed at 37 kDa and 26 kDa. These bands were successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration: 5-10 µg/mL. Data obtained from a previous batch (different goat). This antibody has been successfully used for mouse embryonic stem cells.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Lin, Miller, Contreras et al.: "The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas." in: Cancer research, Vol. 62, Issue 18, pp. 5273-9, 2002 (PubMed).

Alternatives for antigen "Forkhead Box A1 (FOXA1)", type "Antibodies"
Hosts (39), (31), (10)
Reactivities (78), (14), (10), (6), (3), (3), (3), (2), (1)
Applications (62), (47), (44), (17), (6), (3), (3), (1), (1), (1)
Epitopes (14), (8), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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