anti-FOXA1 antibody (Forkhead Box A1) (C-Term)

Details for Product anti-FOXA1 Antibody No. ABIN185021, Supplier: Login to see New
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Antigen
Synonyms
FoxA4a, XFD-1, fkh1, foxa1, pintallavis, xfkh1, xkfh-1, HNF3-alpha, HNF3alpha, Hnf3a, fkh2, tFoxA1, tcf3a, xfkh2, fkh, FOXA1, Ci-fkh, foxa3, Foxa1, fkd7, forkhead-7, hnf3a, HNF3A, TCF3A, HNF-3a, Hnf-3 ... show more
FoxA4a, XFD-1, fkh1, foxa1, pintallavis, xfkh1, xkfh-1, HNF3-alpha, HNF3alpha, Hnf3a, fkh2, tFoxA1, tcf3a, xfkh2, fkh, FOXA1, Ci-fkh, foxa3, Foxa1, fkd7, forkhead-7, hnf3a, HNF3A, TCF3A, HNF-3a, Hnf-3a, Tcf-3a, Tcf3a show less
Epitope
C-Term
(37), (19), (16), (15), (13), (7), (6), (6), (5), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(154), (32), (25), (7), (6), (5), (3), (2), (1), (1), (1)
Host
Goat
(78), (55), (21)
Clonality
Polyclonal
Conjugate
This FOXA1 antibody is un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(116), (72), (54), (33), (26), (11), (10), (9), (7), (7), (6), (3), (3), (2), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
Cross-Reactivity Rat (Rattus), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name FOXA1 / HNF3A (FOXA1 Antibody Abstract)
Background Synonyms: FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha
Gene ID 3169, 15375, 25098
NCBI Accession NP_004487
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 50 kDa band observed in Human lysates of cell line HepG2 (calculated MW of 49.1 kDa according to NP_004487.2). Recommended concentration: 0.2-0.6 µg/mL. Additional bands of unknown identity were also consistently observed at 37 kDa and 26 kDa. These bands were successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration: 5-10 µg/mL. Data obtained from a previous batch (different goat). This antibody has been successfully used for mouse embryonic stem cells.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-FOXA1 antibody (Forkhead Box A1) (C-Term) (ABIN185021) ABIN185021 (0.2µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary...
Background publications Lin, Miller, Contreras et al.: "The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas." in: Cancer research, Vol. 62, Issue 18, pp. 5273-9, 2002 (PubMed).

Catalog No. ABIN185021
$221.76
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