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Forkhead Box A1 (FOXA1) (C-Term) antibody

Details for Product No. ABIN185021
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Antigen
Synonyms
HNF3A, TCF3A, Hnf3a, Tcf3a, Hnf-3a, Tcf-3a, fkd7, forkhead-7, HNF-3a, HNF3-alpha, HNF3alpha, fkh2, foxa1, hnf3a, tFoxA1, tcf3a, FoxA4a, XFD-1, fkh1, pintallavis, xfkh1, xkfh-1, xfkh2, fkh, FOXA1, Ci-f ... show more
HNF3A, TCF3A, Hnf3a, Tcf3a, Hnf-3a, Tcf-3a, fkd7, forkhead-7, HNF-3a, HNF3-alpha, HNF3alpha, fkh2, foxa1, hnf3a, tFoxA1, tcf3a, FoxA4a, XFD-1, fkh1, pintallavis, xfkh1, xkfh-1, xfkh2, fkh, FOXA1, Ci-fkh, foxa3, Foxa1 show less
Epitope
C-Term
(24), (8), (8), (7), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(86), (13), (10), (6), (3), (3), (3), (2), (1)
Host
Goat
(47), (31), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(67), (45), (43), (17), (6), (5), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Shipping to United States (Change)
Availability Available
Catalog No. ABIN185021
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
Cross-Reactivity Rat (Rattus), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name FOXA1 / HNF3A
Background Synonyms: FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha
Gene ID 3169, 15375, 25098
NCBI Accession NP_004487.2
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 50 kDa band observed in Human lysates of cell line HepG2 (calculated MW of 49.1 kDa according to NP_004487.2). Recommended concentration: 0.2-0.6 µg/mL. Additional bands of unknown identity were also consistently observed at 37 kDa and 26 kDa. These bands were successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration: 5-10 µg/mL. Data obtained from a previous batch (different goat). This antibody has been successfully used for mouse embryonic stem cells.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Forkhead Box A1 (FOXA1) (C-Term) antibody ABIN185021 (0.2µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Lin, Miller, Contreras et al.: "The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas." in: Cancer research, Vol. 62, Issue 18, pp. 5273-9, 2002 (PubMed).

Hosts (47), (31), (9)
Reactivities (86), (13), (10), (6), (3), (3), (3), (2), (1)
Applications (67), (45), (43), (17), (6), (5), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1)
Epitopes (24), (8), (8), (7), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185021
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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