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ARAP1 antibody (ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1) (C-Term)

Details for Product anti-ARAP1 Antibody No. ABIN185099, Supplier: Login to see
Antigen
  • CENTD2
  • ARAP1
  • Ci-ArfGAP-8
  • 2410002L19Rik
  • Centd2
  • mKIAA0782
Epitope
C-Term
13
4
3
3
1
1
1
1
1
Reactivity
Mouse (Murine)
21
14
5
2
2
2
1
1
Host
Goat
14
9
7
Clonality
Polyclonal
Conjugate
This ARAP1 antibody is un-conjugated
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
22
17
7
6
6
4
2
Supplier
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Immunogen Peptide with sequence C-AFTADPLSLLRNV, from the C Terminus of the protein sequence according to NP_056057.2, NP_001035207.1, NP_001128662.1.
Blocking Peptide Blocking peptide for this product available: ABIN368791
Specificity This antibody is expected to recognise isoform a (NP_056057.2), isoform c (NP_001035207.1) and isoform d (NP_001128662.1).
Cross-Reactivity Human, Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CENTD2 / ARAP1 (ARAP1 Antibody Abstract)
Background Synonyms: ARAP1, CENTD2, centaurin, delta 2, KIAA0782, ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1, ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1, OTTHUMP00000211462
Gene ID 116985, 69710
NCBI Accession NP_001128662, NP_056057, NP_001035207
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: This antibody showed a 150 kDa in NIH3T3 lysates. Immunocytochemistry: This antibody showed staining concentrated at the ruffles over lamellipodia and focal complexes but not at focal adhesions in NIH3T3 cells.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Miura, Jacques, Stauffer et al.: "ARAP1: a point of convergence for Arf and Rho signaling." in: Molecular cell, Vol. 9, Issue 1, pp. 109-19, 2002 (PubMed).