Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3) antibody

Antigen: Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3)

Synonyms: TIH1, Tih1, zgc:92333, si:dz182n13.5

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185126

Additional Information

Alternative name: PHLDA3 / TIH1

Gene ID: 23612, 27280 (mouse)

Immunogen: C-RARQSLGTGTLVS

Description: All names and symbols: PHLDA3, TIH1, pleckstrin homology-like domain, family A, member 3, pleckstrin homology-like domain, family A, member 2. Official Symbol: PHLDA3. RefSeq number(s): NP_036528. Gene ontology terms: embryogenesis and morphogenesis

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 70kDa band in Human Placenta lysate after 1µg/ml antibody staining. This band was successfully blocked by the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 13.9kDa according to NP_036528. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Frank, Mendelsohn, Ciccone et al.: "A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 10, Issue 12, pp. 1150-9, 2000 (PubMed).