Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 5 (SLC7A5) (C-Term) antibody

Details for Product No. ABIN185255
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Antigen
Synonyms SLC7A5, LAT-1, slc7a5a, LAT1, 4F2LC, CD98, D16S469E, E16, MPE16, hLAT1, TA1, JHI-21, D0H16S474E
Epitope
C-Term
(9), (6), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(44), (16), (16), (12), (12), (12)
Host
Goat
(42), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(20), (20), (10), (8), (6), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185255
374.27 $
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Immunogen Peptide with sequence C-GAPDYENLQELN, from the C Terminus of the protein sequence according to NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1.
Specificity This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LAT1
Background Synonyms: LAT1, LAT, linker for activation of T cells, pp36, 36 kDa phospho-tyrosine adaptor protein, linker for activation of T-cells family member 1
Gene ID 27040
NCBI Accession NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 37 kDa band observed in Human Lymph Node and Peripheral Brood Mononucleocytes lysates and in Mouse and Rat Thymus lysates (calculated MW of 27.9 kDa according to NP_055202.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.01-0.03 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 5 (SLC7A5) (C-Term) antibody ABIN185255 (0.01µg/ml) staining of peripheral blood mononucleocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Finco, Kadlecek, Zhang et al.: "LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway." in: Immunity, Vol. 9, Issue 5, pp. 617-26, 1998 (PubMed).

Validation Images
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