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Catechol-O-Methyltransferase (COMT) (N-Term) antibody

Details for Product No. ABIN185328, Supplier: Log in to see
Antigen
  • COMT
  • mb-comt
  • s-comt
  • comt
  • MGC154444
  • Comt1
  • D16Wsu103e
  • D330014B15Rik
Epitope
N-Term
35
30
7
7
6
6
6
6
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
162
39
37
7
3
3
3
3
2
2
1
1
Host
Goat
120
36
18
1
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
7
2
2
2
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
117
74
42
28
11
11
7
4
3
3
3
1
1
Supplier
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Immunogen Peptide with sequence GDTKEQRILNHVLQC, from the N Terminus of the protein sequence according to NP_000745.1, NP_001128633.1, NP_001128634.1, NP_009294.1.
Blocking Peptide Blocking peptide for this product available: ABIN370145
Specificity This antibody is expected to recognise both reported isoforms. Variants (NP_000745.1, NP_001128633.1, NP_001128634.1) encode the same isoform.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name COMT (COMT Antibody Abstract)
Background Synonyms: COMT, catechol-O-methyltransferase
Gene ID 1312
NCBI Accession NP_001128633, NP_001128634, NP_000745, NP_009294
Pathways
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 26 kDa plus 29 kDa bands observed in Human Testis lysates (calculated MW of 30.0 kDa according to NP_000745.1 and of 24.5 kDa according to NP_009294.1). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Catechol-O-Methyltransferase (COMT) (N-Term) antibody (ABIN185328) Staining of human testis lysate (35µg protein in RIPA buffer) with ABIN185328 (0.2µg/...
Background publications Tunbridge, Harrison, Weinberger: "Catechol-o-methyltransferase, cognition, and psychosis: Val158Met and beyond." in: Biological psychiatry, Vol. 60, Issue 2, pp. 141-51, 2006 (PubMed).

Diatchenko, Slade, Nackley et al.: "Genetic basis for individual variations in pain perception and the development of a chronic pain condition." in: Human molecular genetics, Vol. 14, Issue 1, pp. 135-43, 2004 (PubMed).

Hong, Thompson, Jiang et al.: "Val158Met Polymorphism in catechol-O-methyltransferase gene associated with risk factors for breast cancer." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 12, Issue 9, pp. 838-47, 2003 (PubMed).

Wonodi, Stine, Mitchell et al.: "Association between Val108/158 Met polymorphism of the COMT gene and schizophrenia." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 120B, Issue 1, pp. 47-50, 2003 (PubMed).

Sasaki, Kaneuchi, Sakuragi et al.: "Multiple promoters of catechol-O-methyltransferase gene are selectively inactivated by CpG hypermethylation in endometrial cancer." in: Cancer research, Vol. 63, Issue 12, pp. 3101-6, 2003 (PubMed).