Catechol-O-Methyltransferase (COMT) (N-Term) antibody

Antigen: Catechol-O-Methyltransferase (COMT)

Synonyms: Comt, D16Wsu103e, D330014B15Rik, COMT, mb-comt, s-comt, MGC140449, comt, MGC154444

Epitope: N-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185328

Additional Information

Alternative name: COMT (N Terminus)

Gene ID: 1312

Immunogen: GDTKEQRILNHVLQC

Format: Affinity-purified

Description: All names and symbols: COMT, catechol-O-methyltransferase. Official Symbol: COMT. RefSeq number(s): NP_000745.1, NP_009294.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1) Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx. 26+30kDa bands observed in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and of 24.5kDa according to NP_009294.1). Recommended concentration: 0.2-0.6µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

Staining of human testis lysate (35µg protein in RIPA buffer) with ABIN185328 (0.2µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Sasaki, Kaneuchi, Sakuragi et al.: "Multiple promoters of catechol-O-methyltransferase gene are selectively inactivated by CpG hypermethylation in endometrial cancer." in: Cancer research, Vol. 63, Issue 12, pp. 3101-6, 2003 (PubMed).

Wonodi, Stine, Mitchell et al.: "Association between Val108/158 Met polymorphism of the COMT gene and schizophrenia." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 120B, Issue 1, pp. 47-50, 2003 (PubMed).

Hong, Thompson, Jiang et al.: "Val158Met Polymorphism in catechol-O-methyltransferase gene associated with risk factors for breast cancer." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 12, Issue 9, pp. 838-47, 2003 (PubMed).

Diatchenko, Slade, Nackley et al.: "Genetic basis for individual variations in pain perception and the development of a chronic pain condition." in: Human molecular genetics, Vol. 14, Issue 1, pp. 135-43, 2004 (PubMed).

Tunbridge, Harrison, Weinberger: "Catechol-o-methyltransferase, cognition, and psychosis: Val158Met and beyond." in: Biological psychiatry, Vol. 60, Issue 2, pp. 141-51, 2006 (PubMed).