COMT antibody

Catalog No. ABIN2854980

Product Details anti-COMT Antibody

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COMT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to COMT (catechol-O-methyltransferase)
COMT antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human COMT. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse spleen , Molt-4 , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.32 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-COMT antibody (ABIN2854980)

Ikeda, Iijima, Shinoda, Iwamoto, Takeda: "Inhibitory Effect of Bovine Lactoferrin on Catechol-O-Methyltransferase." in: Molecules (Basel, Switzerland), Vol. 22, Issue 8, (2018) (PubMed).

Target Details for COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Alternative Name: catechol-O-methyltransferase (COMT Products)

Synonyms: Comt1 antibody, D16Wsu103e antibody, D330014B15Rik antibody, COMT antibody, mb-comt antibody, s-comt antibody, comt antibody, MGC154444 antibody, zgc:114157 antibody, si:dkey-13a21.15 antibody, zK13A21.15 antibody, zgc:162236 antibody, catechol-O-methyltransferase antibody, microRNA 4761 antibody, catechol-O-methyltransferase S homeolog antibody, methyltransferase antibody, catechol-o-methyltransferase antibody, catechol-O-methyltransferase a antibody, catechol-O-methyltransferase b antibody, catechol O-methyltransferase antibody, COMT antibody, Comt antibody, MIR4761 antibody, comt antibody, comt.2 antibody, comt.S antibody, Rv1703c antibody, Mb1729c antibody, comta antibody, comtb antibody, LOC100354142 antibody

Background: Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.

Cellular Localization: Isoform Soluble: Cytoplasm , Isoform Membrane-bound: Cell membrane

Molecular Weight: 30 kDa

Gene ID: 1312

UniProt: P21964

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome