Reticulon 4 Receptor (RTN4R) (Internal Region) antibody

Details for Product No. ABIN185403
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Synonyms NgR, NGR, NOGOR, NgR1, Nogor
Internal Region
(7), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1)
(39), (15), (10), (3), (2), (1), (1)
(22), (15), (4), (3)
(3), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(38), (20), (9), (2), (1)
Pubmed 2 references available
Quantity 100 μg
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Immunogen Peptide with sequence C-LQALPDDTFRDLGN, from the internal region of the protein sequence according to NP_075380.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Pig (Porcine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Nogo 66 receptor (RTN4R Antibody Abstract)
Background Synonyms: Nogo-66 receptor, RTN4R, reticulon 4 receptor, HGNC:1860, NGR, NOGOR, UNQ330/PRO526, nogo receptor, reticulon 4 receptor precursor, OTTHUMP00000197866
Gene ID 65078
NCBI Accession NP_075380
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Experiments gave bands at approx 90-100 kDa and 37+40+45 kDa in Human brain lysate after 0.3 µg/mL antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1,22(13):5505-15). This protein has a calculated MW of 50.7 kDa according to NP_075380.1. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-Reticulon 4 Receptor (RTN4R) (Internal Region) antibody (ABIN185403) ABIN185403 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) wi...
Background publications Shao, Browning, Lee et al.: "TAJ/TROY, an orphan TNF receptor family member, binds Nogo-66 receptor 1 and regulates axonal regeneration." in: Neuron, Vol. 45, Issue 3, pp. 353-9, 2005 (PubMed).

Trifunovski, Josephson, Ringman et al.: "Neuronal activity-induced regulation of Lingo-1." in: Neuroreport, Vol. 15, Issue 15, pp. 2397-400, 2005 (PubMed).

Catalog No. ABIN185403
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