RTN4R antibody (Extracellular)

Catalog No. ABIN7886729

The Rabbit Polyclonal anti-RTN4R antibody (ABIN7886729) specifically detects RTN4R in WB, IHC, FACS, IF, IC and LCI. The antibody is reactive with Human samples.

Quick Overview for RTN4R antibody (Extracellular) (ABIN7886729)

Target: RTN4R (Reticulon 4 Receptor (RTN4R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RTN4R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-RTN4R Antibody

Binding Specificity: AA 366-381, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Nogo Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 15,16 amino acid residues identical

Characteristics: Anti-Nogo Receptor (extracellular) Antibody is directed against an extracellular epitope of the human Nogo receptor. Anti-Nogo Receptor (extracellular) Antibody (ABIN7043601, ABIN7044757 and ABIN7044758) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NgR from human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7236730)

Blocking Peptide: (ABIN7236730)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for RTN4R

Target: RTN4R (Reticulon 4 Receptor (RTN4R))

Alternative Name: RTN4R

Background:

Synonyms: NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Description: The Nogo receptor is a leucine-rich repeat (LRR) containing protein with a glycosylphosphatidylinositol (GPI) anchored C-terminus. The receptor was identified on the basis of its ability to bind with high affinity to Nogo-A a member of the reticulon family expressed in the cell membrane of oligodendrocytes.1Nogo-A attracted attention when it was demonstrated that it is a myelin protein capable of inhibiting axonal growth following nerve injury.2,3 Other myelin proteins also identified as axonal growth inhibitors are the myelin-associated glycoprotein (MAG) a sialic-dependent immunoglobulin-like family member lectin (SIGLEC) and the oligodendrocyte myelin glycoprotein (OmgP) which is a GPI-anchored membrane protein. Remarkably, despite their structural diversity, all three axonal growth inhibitors bind to the Nogo receptor with high affinity.4,5Since the Nogo receptor is a GPI-anchored protein it was expected that it would require another protein component to transduce the Nogo-A binding information into the responding neurons interior. Indeed, it was shown that the neurotrophin receptor p75 (p75NTR) interacts with Nogo receptor and a third protein termed LINGO-1 to mediate axonal growth inhibition signaling.6,7

Gene ID: 65078

NCBI Accession: NM_023004

UniProt: Q9BZR6

Pathways: Neurotrophin Signaling Pathway