RTN4R antibody (Extracellular) (FITC)

Catalog No. ABIN7043602

This Rabbit Polyclonal antibody specifically detects RTN4R in FACS. It exhibits reactivity toward Human.

Quick Overview for RTN4R antibody (Extracellular) (FITC) (ABIN7043602)

Target: RTN4R (Reticulon 4 Receptor (RTN4R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RTN4R antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-RTN4R Antibody (FITC)

Binding Specificity: AA 366-381, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Nogo Receptor Conjugated to the Fluorescent Dye FITC

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 15,16 amino acid residues identical

Characteristics: Anti-Nogo Receptor (extracellular) Antibody (ABIN7043601, ABIN7044757 and ABIN7044758) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NgR1 from human, mouse and rat samples. \nAnti-Nogo Receptor (extracellular)-FITC Antibody (ABIN7043601, ABIN7044757 and ABIN7044758)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236730)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for RTN4R

Target: RTN4R (Reticulon 4 Receptor (RTN4R))

Alternative Name: RTN4R

Background:

Synonyms: NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Description: Neurite outgrowth inhibitor receptor 1 (NgR1, Nogo Receptor) is a glycosylphosphatidylinositol (GPI) transmembrane protein expressed on the surface of axons in the CNS. Its key binding domain is an extracellular leucine-rich repeat domain that interacts with Myelin-associated inhibitory factors (MAIFs) composed of MAG, Neurite outgrowth inhibitor (Nogo)-A, and OMgp1,2.NgR1 binds to Neurite outgrowth inhibitor (Nogo) through a short hydrophilic loop between two hydrophobic domains. This binding activates intracellular molecular switches through the heteromerization with NgR1 co-receptors, p75NTR and LINGO-1.This process facilitates neurite outgrowth dynamics, it also governs the plasticity of the CNS at the axo-dendritic and axo-glial levels3.During CNS neurodegenerative disorders and in animal models of inflammatory demyelination, MAIFs, containing Nogo, accumulate and therefore there is an increased binding to their cognate receptor. This marks Nogo-NgR1 interaction as a potential target of new therapeutics for neurodegenerative diseases4.

Gene ID: 65078

NCBI Accession: NM_023004

UniProt: Q9BZR6

Pathways: Neurotrophin Signaling Pathway