Neuropeptide Y Receptor Y5 (NPY5R) (Internal Region) antibody

Details for Product No. ABIN185409
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Antigen
Synonyms MGC146705, NPY5-R, NPY5R, NPYY5-R, NPYR5, NPY5, Y5R
Epitope
Internal Region
(9), (4), (3), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(49), (19), (17), (13), (13)
Host
Goat
(44), (4), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(24), (18), (17), (17), (16), (11), (10), (7), (1)
Pubmed 2 references available
Quantity 100 μg
Options
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Catalog No. ABIN185409
374.27 $
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Immunogen Peptide with sequence C-GLSNKENRLEEN, from the internal region of the protein sequence according to NP_006165.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Neuropeptide Y receptor Y5
Background Synonyms: neuropeptide Y receptor Y5, HGNC:7958, NPY5R, NPYR5
Molecular Weight 50.7kDa
Gene ID 4889
NCBI Accession NP_006165
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50 kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7 kDa according to NP_006165.1). Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration: 4-6 µg/mL. Immunoprecipitation: Customer feedback showed specific results when this product was used in IP.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Neuropeptide Y Receptor Y5 (NPY5R) (Internal Region) antibody ABIN185409 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product cited in: Sheriff, Ali, Yahya et al.: "Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line." in: Molecular cancer research : MCR, Vol. 8, Issue 4, pp. 604-14, 2010 (PubMed).

General Badman, Flier: "The gut and energy balance: visceral allies in the obesity wars." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1909-14, 2005 (PubMed).

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