Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibody

Details for Product No. ABIN185581
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Antigen
Synonyms
Ago, Ago-1, Ago1, CG6671, Dm Ago1, Dmel\\CG6671, MRE20, ago1, ago1-1, anon-WO0257455.29, dAGO1, dAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121, GB12654, dsim_GLEANR_9718, DsimGD25729, GD25729, EIF ... show more
Ago, Ago-1, Ago1, CG6671, Dm Ago1, Dmel\\CG6671, MRE20, ago1, ago1-1, anon-WO0257455.29, dAGO1, dAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121, GB12654, dsim_GLEANR_9718, DsimGD25729, GD25729, EIF2C1, EIF2C, GERP95, Q99, Eif2c1, ARGONAUTE 1, T1N15.2, T1N15_2 show less
Epitope
Internal Region
(5), (3)
Reactivity
Human
(29), (17), (16), (11), (11), (11), (3), (1)
Host
Goat
(24), (5), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(19), (9), (8), (8), (5), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185581
374.27 $
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Immunogen Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.
Specificity This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name EIF2C1
Background Synonyms: EIF2C1, eukaryotic translation initiation factor 2C, 1, AGO1, EIF2C, GERP95, Q99, Golgi Endoplasmic Reticulum protein 95 kDa, argonaute 1, putative RNA-binding protein Q99
Gene ID 26523
NCBI Accession NP_036331
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing human EIF2C1bands of approx. 110 kDa and 35 kDa are observed. The 110 kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2 kDa according to NP_036331.1. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibody HEK293 overexpressing EIF2C1 and probed with ABIN185581 (non-transfected HEK293 in lane B).
General Gregory, Chendrimada, Cooch et al.: "Human RISC couples microRNA biogenesis and posttranscriptional gene silencing." in: Cell, Vol. 123, Issue 4, pp. 631-40, 2005 (PubMed).

Validation Images
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