AGO1 antibody (N-Term)

Catalog No. ABIN488536

This Rabbit Polyclonal antibody specifically detects AGO1 in WB, IF, ChIP and IL. It exhibits reactivity toward Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth) and Nicotiana benthamiana.It has been mentioned in 1 publication

Quick Overview for AGO1 antibody (N-Term) (ABIN488536)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivity: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth), Nicotiana benthamiana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunolocalization (IL)

Product Details anti-AGO1 Antibody

Binding Specificity: N-Term

Purpose: Anti-AGO1 | Argonaute 1

Specificity: Antibody binds microRNA and tasiRNAs, preference for 21ntmiRNAs with 5'U

Cross-Reactivity (Details): Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana
Not reactive in: Chlamydomonas reinhardtii, Medicago sativa, Salvia sp., Spinacia oleracea, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea maysMammals

Predicted Reactivity: Brassica pekinensis,Capsella rubella,Cucumis sativus,Glycine max,Malus domestica,Pisum sativum,Ricinus communis,Solanum lycopersicum,Solanum tuberosum,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated

Application Details

Application Notes: 2 μg (ChIP), 1: 200 (IF), (IL), small-RNA-IP-Seq, 1 : 5000-1 : 10 000 (WB)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-AGO1 antibody (ABIN488536)

Speth, Willing, Rausch, Schneeberger, Laubinger: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: AGO1

Background:

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Additional information: Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.To detect AGO1 in Nicotiana benthamiana, please inquire.Recommended for detection of AGO1: extreme low femtogram range chemiluminescent detection reagent

Molecular Weight: 116.4 | 130 kDa

Gene ID: 841262

UniProt: O04379

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization