Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody

Details for Product No. ABIN185634
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Antigen
Synonyms Ci-Wnt3, WNT3, int4, Xwnt3, wnt-3, wnt3l, Xwnt-3, wnt3, Int-4, Wnt-3, INT4, wnt3-A
Epitope
Internal Region
(12), (7), (2), (1), (1)
Reactivity
Human, Mouse (Murine)
(26), (5), (5), (4)
Host
Goat
(15), (6), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(27), (13), (8), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 100 μg
Options
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Catalog No. ABIN185634
374.27 $
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Immunogen Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name WNT3
Background Synonyms: WNT3, wingless-type MMTV integration site family, member 3, INT4, MGC131950, MGC138321, MGC138323, WNT-3 proto-oncogene protein precursor
Gene ID 7473, 22415, 24882
NCBI Accession NP_110380
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows strong fibrous staining in myoepithelial cells. Data obtained from a previous batch (different goat). Recommended concentration: 3-5 µg/mL. Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J. 2011 Jul 11, PMID: 21746862.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody ABIN185634 (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody (2) ABIN185634 (1 µg/mL) staining of lysate of cell line HEK293 (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody (3) ABIN185634 (3.8 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody (4) ABIN185634 (2 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Product cited in: Okamoto, Inoue, Iwamura et al.: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, 2011 (PubMed).

General Lie, Colamarino, Song et al.: "Wnt signalling regulates adult hippocampal neurogenesis." in: Nature, Vol. 437, Issue 7063, pp. 1370-5, 2005 (PubMed).

Schmitt, Shi, Wolf et al.: "Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping." in: Nature, Vol. 439, Issue 7072, pp. 31-7, 2006 (PubMed).

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