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Details for Product No. ABIN185634

Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody

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Antigen
Synonyms INT4, Int-4, Wnt-3, Xwnt-3, Xwnt3, wnt-3, wnt3-A, wnt3l, Ci-Wnt3, WNT3, int4, wnt3
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human, Mouse (Murine)
Host
Clonality Polyclonal
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Catalog no. ABIN185634
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Availability Ships within 5 Business Days
Immunogen Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name WNT3
Background Synonyms: WNT3, wingless-type MMTV integration site family, member 3, INT4, MGC131950, MGC138321, MGC138323, WNT-3 proto-oncogene protein precursor
Gene ID 7473, 22415, 24882
NCBI Accession NP_110380.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows strong fibrous staining in myoepithelial cells. Data obtained from a previous batch (different goat). Recommended concentration: 3-5 µg/mL. Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J. 2011 Jul 11, PMID: 21746862.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Okamoto, Inoue, Iwamura et al.: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, 2011 (PubMed).

Lie, Colamarino, Song et al.: "Wnt signalling regulates adult hippocampal neurogenesis." in: Nature, Vol. 437, Issue 7063, pp. 1370-5, 2005 (PubMed).

Schmitt, Shi, Wolf et al.: "Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping." in: Nature, Vol. 439, Issue 7072, pp. 31-7, 2006 (PubMed).

Alternatives for antigen "Wingless-Type MMTV Integration Site Family, Member 3 (WNT3)", type "Antibodies"
Hosts (10), (7), (6)
Reactivities (20), (5), (5), (4)
Applications (21), (8), (6), (1), (1), (1), (1)
Epitopes (6), (6), (2), (1), (1), (1), (1)
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