Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody

Details for Product No. ABIN185634
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Antigen
Synonyms INT4, Int-4, Wnt-3, Xwnt-3, Xwnt3, wnt-3, wnt3-A, wnt3l, Ci-Wnt3, WNT3, int4, wnt3
Epitope
Internal Region
(5), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(19), (7), (5), (4)
Host
Goat
(10), (8), (6)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(22), (11), (10), (5), (4), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN185634
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name WNT3
Background Synonyms: WNT3, wingless-type MMTV integration site family, member 3, INT4, MGC131950, MGC138321, MGC138323, WNT-3 proto-oncogene protein precursor
Gene ID 7473, 22415, 24882
NCBI Accession NP_110380.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows strong fibrous staining in myoepithelial cells. Data obtained from a previous batch (different goat). Recommended concentration: 3-5 µg/mL. Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J. 2011 Jul 11, PMID: 21746862.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody ABIN185634 (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody (2) ABIN185634 (1 µg/mL) staining of lysate of cell line HEK293 (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody (3) ABIN185634 (3.8 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody (4) ABIN185634 (2 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Product cited in: Okamoto, Inoue, Iwamura et al.: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, 2011 (PubMed).

General Lie, Colamarino, Song et al.: "Wnt signalling regulates adult hippocampal neurogenesis." in: Nature, Vol. 437, Issue 7063, pp. 1370-5, 2005 (PubMed).

Schmitt, Shi, Wolf et al.: "Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping." in: Nature, Vol. 439, Issue 7072, pp. 31-7, 2006 (PubMed).

Hosts (10), (8), (6)
Reactivities (19), (7), (5), (4)
Applications (22), (11), (10), (5), (4), (1), (1), (1)
Epitopes (5), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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