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WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region)

Details for Product anti-WNT3 Antibody No. ABIN185634, Supplier: Login to see
Antigen
  • Ci-Wnt3
  • WNT3
  • int4
  • Xwnt3
  • wnt-3
  • wnt3l
  • Xwnt-3
  • wnt3
  • Int-4
  • Wnt-3
  • INT4
  • wnt3-A
Alternatives
anti-Human WNT3 antibody for ELISA
Epitope
Internal Region
29
12
10
10
8
3
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
70
12
9
6
1
1
1
1
Host
Goat
33
19
18
Clonality
Polyclonal
Conjugate
This WNT3 antibody is un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
63
34
17
13
11
8
3
2
1
Supplier
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Immunogen Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
Blocking Peptide Blocking peptide for this product available: ABIN369133
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name WNT3 (WNT3 Antibody Abstract)
Background Synonyms: WNT3, wingless-type MMTV integration site family, member 3, INT4, MGC131950, MGC138321, MGC138323, WNT-3 proto-oncogene protein precursor
Gene ID 7473, 22415, 24882
NCBI Accession NP_110380
Pathways WNT Signaling
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows strong fibrous staining in myoepithelial cells. Data obtained from a previous batch (different goat). Recommended concentration: 3-5 µg/mL. Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J. 2011 Jul 11, PMID: 21746862.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN185634) ABIN185634 (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). ...
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN185634) ABIN185634 (1 µg/mL) staining of lysate of cell line HEK293 (35 µg protein in RIPA bu...
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN185634) ABIN185634 (3.8 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen re...
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN185634) ABIN185634 (2 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retr...
Product cited in: Fujimaki, Hidaka, Asashima et al.: "Wnt protein-mediated satellite cell conversion in adult and aged mice following voluntary wheel running." in: The Journal of biological chemistry, Vol. 289, Issue 11, pp. 7399-412, 2014 (PubMed).

Okamoto, Inoue, Iwamura et al.: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, 2011 (PubMed).

Background publications Schmitt, Shi, Wolf et al.: "Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping." in: Nature, Vol. 439, Issue 7072, pp. 31-7, 2006 (PubMed).

Lie, Colamarino, Song et al.: "Wnt signalling regulates adult hippocampal neurogenesis." in: Nature, Vol. 437, Issue 7063, pp. 1370-5, 2005 (PubMed).