FAS antibody (PE)

Catalog No. ABIN187287

Product Details anti-FAS Antibody (PE)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 3-22

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry Immunofluorescence PBMC: Add 10 µL of MAB/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 min. at 4 °C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Whole Blood: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 min. at RT. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for lysed whole blood and immunofluorescence analysis with a flow cytometer or microscope. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: Antibody supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for RPE.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Background: Activation of either the 55-kD tumor necrosis factor receptor (TNF-R1) or CD95 (Fas/Apo-1) causes apoptosis of cells and liver failure in mice, and has been associated with human liver disorders.

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity