TBC1 Domain Family, Member 4 (TBC1D4) (Internal Region) antibody

Details for Product No. ABIN190878
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Antigen
Synonyms AS160, 5930406J04Rik, A930035N22, AV295684, As160, RGD1561609
Epitope
Internal Region
(8), (7), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(58), (21), (14), (12), (12), (2), (1)
Host
Goat
(48), (7), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(37), (25), (18), (13), (13), (11), (10), (3), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN190878
374.27 $
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Immunogen Peptide with sequence C-DDPEKIEERKKSK, from the internal region of the protein sequence according to NP_055647.2.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name AS160 / TBC1D4
Background Synonyms: TBC1D4, AS160, TBC1 domain family, member 4, RP11-159J2.1, DKFZp779C0666, Acrg embryonic lethality (mouse) minimal region ortholog, OTTHUMP00000040818, TBC (Tre-2, BUB2, CDC16) domain-containing protein, KIAA0603
Gene ID 9882
NCBI Accession NP_055647
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 150 kDa band observed in lysates of cell lines Daudi and MOLT4 (calculated MW of 147 kDa according to NP_055647.2). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Karlsson, Zierath, Kane et al.: "Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects." in: Diabetes, Vol. 54, Issue 6, pp. 1692-7, 2005 (PubMed).

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