TBC1D4 antibody (pThr642)

Catalog No. ABIN1531299

This Rabbit Polyclonal antibody specifically detects TBC1D4 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for TBC1D4 antibody (pThr642) (ABIN1531299)

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TBC1D4 Antibody

Binding Specificity: AA 611-660, pThr642

Purpose: TBC1D4 (phospho Thr642) Antibody

Specificity: Phospho-TBC1D4 (T642) Polyclonal Antibody detects endogenous levels of TBC1D4 protein only when phosphorylated at T642.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AS160 around the phosphorylation site of Thr642. AA range:611-660

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: AS160

Background:

Synonyms: TBC1D4, AS160, KIAA0603, TBC1 domain family member 4, Akt substrate of 160 kDa, AS160

Background: This gene is a member of the Tre-2/BUB2/CDC16 domain family.The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Ph

Gene Name: TBC1D4

Protein Name: TBC1 domain family member 4

Molecular Weight: 146 kDa

Gene ID: 9882

OMIM: 612465

UniProt: O60343