EPH Receptor A2 antibody (Extracellular Domain)

Catalog No. ABIN204192

Product Details anti-EPH Receptor A2 Antibody

Target: EPH Receptor A2 (EPHA2)

Binding Specificity: Extracellular Domain

Reactivity: Human, Mouse, Dog, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity: Recognizes human EphA2.

Purification: Protein G purified

Immunogen: NS0-derived rhEphA2 extracellular domain.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (25 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 μg/mL. Add 10 μL to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 μg/mL to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 200 μL sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handling Advice: Avoid frequent freeze thaws. Store undiluted.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -70°C. Stable for 1 year from date of receipt.

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EPHA2 / EPH Receptor A2 (EPHA2 Products)

Background: Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1

Gene ID: 1969

UniProt: P29317

Pathways: RTK Signaling