SIRPA antibody

Catalog No. ABIN213153

Product Details anti-SIRPA Antibody

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes rat CD172a also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. ED9 binds to a different epitope on SIRP than MRC OX-41 and has been reported to block the interaction of CD172a - CD47. Removal of Sodium Azide is recommended prior to use in functional assays. XXX recommend the use of EQU003 for this purpose.

Purification: Protein A purified

Immunogen: Spleen cell homogenate.

Clone: ED9

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:10 - 1:50), IHC, IHC-Fr (1:100 - 1:1000), IP, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172A / SIRPA (SIRPA Products)

Background: Name/Gene ID: SIRPA

Synonyms: SIRPA, CD172A, CD172a antigen, Inhibitory receptors shps-1, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, p84, SHPS-1, SHPS1, SIRP, Sirp-alpha-1, Sirp-alpha-3, Sirp-alpha1, MYD1, SIRPalpha, SIRPalpha2, BIT, MFR, MYD-1, PTPNS1, SHP substrate 1, Sirp-alpha-2

Gene ID: 140885